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https://hdl.handle.net/10316/100810
Título: | In situ electrochemical investigation of the interaction between bacteria Xylella fastidiosa DNA and copper(II) using DNA-electrochemical biosensors | Autor: | Machini, W.B.S. Oliveira-Brett, A.M. |
Palavras-chave: | Bacterial DNA; DNA-electrochemical biosensor; Pesticide-DNA interaction; Xylella fastidiosa | Data: | 2021 | Projeto: | UID/EMS/00285/2020 UIDB/00285/2020 PhD Grant 232296/2014-6 (W.B.S. Machini) CNPq/Brazil |
Título da revista, periódico, livro ou evento: | Electrochemistry Communications | Volume: | 125 | Resumo: | New emerging bacterial plant diseases are spreading fast in different geographical regions, and effective safety measures have not been found. Xylella fastidiosa (Xf) is a Gram-negative bacterial plant pathogen classified as a priority quarantine pest, which affects economically important agricultural crops. Although there is no treatment for diseases caused by Xf, copper-based compounds are widely applied to its hosts. A Xf-DNA-electrochemical biosensor based on bacterial DNA from Xf, is reported here for the first time, and the Cu(II)-Xf-dsDNA interaction was investigated in situ. The Cu(II)-DNA interaction was also evaluated using ctdsDNA-, poly[G]- and poly[A]-electrochemical biosensors. The Cu(II)-Xf-dsDNA interaction occurs by Cu(II) binding at different sites, independent of the bacterial DNA sequence, leading to the condensation/aggregation of Xf-DNA strands due to the formation of a rigid Cu(II)-Xf-dsDNA complex structure. Cu(II) did not cause oxidative DNA damage. Resistance of the bacterial Xf-dsDNA to Cu(II) was observed. | URI: | https://hdl.handle.net/10316/100810 | ISSN: | 13882481 | DOI: | 10.1016/j.elecom.2021.106975 | Direitos: | openAccess |
Aparece nas coleções: | I&D CEMMPRE - Artigos em Revistas Internacionais |
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1-s2.0-S138824812100059X-main.pdf | 572.07 kB | Adobe PDF | Ver/Abrir |
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