Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/100810
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Machini, W.B.S. | - |
dc.contributor.author | Oliveira-Brett, A.M. | - |
dc.date.accessioned | 2022-07-12T14:04:17Z | - |
dc.date.available | 2022-07-12T14:04:17Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 13882481 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/100810 | - |
dc.description.abstract | New emerging bacterial plant diseases are spreading fast in different geographical regions, and effective safety measures have not been found. Xylella fastidiosa (Xf) is a Gram-negative bacterial plant pathogen classified as a priority quarantine pest, which affects economically important agricultural crops. Although there is no treatment for diseases caused by Xf, copper-based compounds are widely applied to its hosts. A Xf-DNA-electrochemical biosensor based on bacterial DNA from Xf, is reported here for the first time, and the Cu(II)-Xf-dsDNA interaction was investigated in situ. The Cu(II)-DNA interaction was also evaluated using ctdsDNA-, poly[G]- and poly[A]-electrochemical biosensors. The Cu(II)-Xf-dsDNA interaction occurs by Cu(II) binding at different sites, independent of the bacterial DNA sequence, leading to the condensation/aggregation of Xf-DNA strands due to the formation of a rigid Cu(II)-Xf-dsDNA complex structure. Cu(II) did not cause oxidative DNA damage. Resistance of the bacterial Xf-dsDNA to Cu(II) was observed. | pt |
dc.language.iso | eng | pt |
dc.relation | UID/EMS/00285/2020 | pt |
dc.relation | UIDB/00285/2020 | pt |
dc.relation | PhD Grant 232296/2014-6 (W.B.S. Machini) CNPq/Brazil | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | Bacterial DNA | pt |
dc.subject | DNA-electrochemical biosensor | pt |
dc.subject | Pesticide-DNA interaction | pt |
dc.subject | Xylella fastidiosa | pt |
dc.title | In situ electrochemical investigation of the interaction between bacteria Xylella fastidiosa DNA and copper(II) using DNA-electrochemical biosensors | pt |
dc.type | article | - |
degois.publication.firstPage | 106975 | pt |
degois.publication.title | Electrochemistry Communications | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1016/j.elecom.2021.106975 | pt |
degois.publication.volume | 125 | pt |
dc.date.embargo | 2021-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.project.grantno | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/EMS/00285/2020/PT/Centre for Mechanical Engineering | - |
crisitem.project.grantno | Centre for Mechanical Enginnering, Materials and Processes | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0002-6244-0891 | - |
Appears in Collections: | I&D CEMMPRE - Artigos em Revistas Internacionais |
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1-s2.0-S138824812100059X-main.pdf | 572.07 kB | Adobe PDF | View/Open |
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