Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27278
Title: Chitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healing
Authors: Moura, Liane I. F. 
Dias, Ana M. A. 
Leal, Ermelindo C. 
Carvalho, Lina 
Sousa, Hermínio C. de 
Carvalho, Eugénia 
Keywords: Chitosan derivatives; Wound dressings; Diabetic foot ulcers; Neurotensin; Wound healing
Issue Date: Feb-2014
Publisher: Elsevier
Citation: MOURA, Liane I. F. [et. al] - Chitosan-based dressings loaded with neurotensin—an efficient strategy to improve early diabetic wound healing. "Acta Biomaterialia". ISSN 1742-7061. Vol. 10 Nº. 2 (2014) p. 843-857
Abstract: One important complication of diabetes mellitus is chronic, non-healing diabetic foot ulcers (DFUs). This study aims to develop and use dressings based on chitosan derivatives for the sustained delivery of neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing. Three different derivatives, namely N-carboxymethyl chitosan, 5-methyl pyrrolidinone chitosan (MPC) and N-succinyl chitosan, are presented as potential biomaterials for wound healing applications. Our results show that MPC has the best fluid handling capacity and delivery profile, also being non-toxic to Raw 264.7 and HaCaT cells. NT-loaded and non-loaded MPC dressings were applied to control/diabetic wounds to evaluate their in vitro/in vivo performance. The results show that the former induced more rapid healing (50% wound area reduction) in the early phases of wound healing in diabetic mice. A NT-loaded MPC foam also reduced expression of the inflammatory cytokine TNF-α (P < 0.001) and decreased the amount of inflammatory infiltrate on day 3. On day 10 MMP-9 was reduced in diabetic skin (P < 0.001), significantly increasing fibroblast migration and collagen (COL1A1, COL1A2 and COL3A1) expression and deposition. These results suggest that MPC-based dressings may work as an effective support for sustained NT release to reduce DFUs.
URI: http://hdl.handle.net/10316/27278
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2013.09.040
Rights: openAccess
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
FCTUC Eng.Química - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat 
Chitosan-based dressings loaded with neurotensin-an efficient strategy.pdf1.01 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

55
checked on Apr 12, 2019

WEB OF SCIENCETM
Citations

54
checked on Jun 11, 2019

Page view(s) 20

430
checked on Jun 11, 2019

Download(s) 50

275
checked on Jun 11, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.