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https://hdl.handle.net/10316/113762
Título: | Glycopolymers Mediate Suicide Gene Therapy in ASGPR-Expressing Hepatocellular Carcinoma Cells in Tandem with Docetaxel | Autor: | Santo, Daniela Cordeiro, Rosemeyre A. Mendonça, Patrícia V. Serra, Arménio Coelho, Jorge Faneca, Henrique |
Data: | 13-Mar-2023 | Editora: | American Chemical Society | Projeto: | IF/01007/2015 POCI-01-0145-FEDER-30916 UIDB/04539/2020 UIDP/04539/2020 SFRH/BD/132601/2017 CEECIND.00186.2020 REEQ/481/QUI/2006 QFI/0168/2012 CENTRO-07-CT62-FEDER- 002012 |
Título da revista, periódico, livro ou evento: | Biomacromolecules | Volume: | 24 | Número: | 3 | Resumo: | Cationic glycopolymers stand out as gene delivery nanosystems due to their inherent biocompatibility and high binding affinity to the asialoglycoprotein receptor (ASGPR), a target receptor overexpressed in hepatocellular carcinoma (HCC) cells. However, their synthesis procedure remains laborious and complex, with problems of solubilization and the need for protection/deprotection steps. Here, a mini-library of well-defined poly(2-aminoethyl methacrylate hydrochloride-co-poly(2-lactobionamidoethyl methacrylate) (PAMA-co-PLAMA) glycopolymers was synthesized by activators regenerated by electron transfer (ARGET) ATRP to develop an efficient gene delivery nanosystem. The glycoplexes generated had suitable physicochemical properties and showed high ASGPR specificity and high transfection efficiency. Moreover, the HSV-TK/GCV suicide gene therapy strategy, mediated by PAMA144-co-PLAMA19-based nanocarriers, resulted in high antitumor activity in 2D and 3D culture models of HCC, which was significantly enhanced by the combination with small amounts of docetaxel. Overall, our results demonstrated the potential of primary-amine polymethacrylate-containing-glycopolymers as HCC-targeted suicide gene delivery nanosystems and highlight the importance of combined strategies for HCC treatment. | URI: | https://hdl.handle.net/10316/113762 | ISSN: | 1525-7797 1526-4602 |
DOI: | 10.1021/acs.biomac.2c01329 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Eng.Química - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D CEMMPRE - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Glycopolymers-Mediate-Suicide-Gene-Therapy-in-ASGPRExpressing-Hepatocellular-Carcinoma-Cells-in-Tandem-with-DocetaxelBiomacromolecules.pdf | 10.33 MB | Adobe PDF | Ver/Abrir |
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