Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/113762
DC Field | Value | Language |
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dc.contributor.author | Santo, Daniela | - |
dc.contributor.author | Cordeiro, Rosemeyre A. | - |
dc.contributor.author | Mendonça, Patrícia V. | - |
dc.contributor.author | Serra, Arménio | - |
dc.contributor.author | Coelho, Jorge | - |
dc.contributor.author | Faneca, Henrique | - |
dc.date.accessioned | 2024-03-01T09:47:18Z | - |
dc.date.available | 2024-03-01T09:47:18Z | - |
dc.date.issued | 2023-03-13 | - |
dc.identifier.issn | 1525-7797 | pt |
dc.identifier.issn | 1526-4602 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/113762 | - |
dc.description.abstract | Cationic glycopolymers stand out as gene delivery nanosystems due to their inherent biocompatibility and high binding affinity to the asialoglycoprotein receptor (ASGPR), a target receptor overexpressed in hepatocellular carcinoma (HCC) cells. However, their synthesis procedure remains laborious and complex, with problems of solubilization and the need for protection/deprotection steps. Here, a mini-library of well-defined poly(2-aminoethyl methacrylate hydrochloride-co-poly(2-lactobionamidoethyl methacrylate) (PAMA-co-PLAMA) glycopolymers was synthesized by activators regenerated by electron transfer (ARGET) ATRP to develop an efficient gene delivery nanosystem. The glycoplexes generated had suitable physicochemical properties and showed high ASGPR specificity and high transfection efficiency. Moreover, the HSV-TK/GCV suicide gene therapy strategy, mediated by PAMA144-co-PLAMA19-based nanocarriers, resulted in high antitumor activity in 2D and 3D culture models of HCC, which was significantly enhanced by the combination with small amounts of docetaxel. Overall, our results demonstrated the potential of primary-amine polymethacrylate-containing-glycopolymers as HCC-targeted suicide gene delivery nanosystems and highlight the importance of combined strategies for HCC treatment. | pt |
dc.language.iso | eng | pt |
dc.publisher | American Chemical Society | pt |
dc.relation | IF/01007/2015 | pt |
dc.relation | POCI-01-0145-FEDER-30916 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | UIDP/04539/2020 | pt |
dc.relation | SFRH/BD/132601/2017 | pt |
dc.relation | CEECIND.00186.2020 | pt |
dc.relation | REEQ/481/QUI/2006 | pt |
dc.relation | QFI/0168/2012 | pt |
dc.relation | CENTRO-07-CT62-FEDER- 002012 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Docetaxel | pt |
dc.subject.mesh | Asialoglycoprotein Receptor | pt |
dc.subject.mesh | Cell Line, Tumor | pt |
dc.subject.mesh | Genetic Therapy | pt |
dc.subject.mesh | Carcinoma, Hepatocellular | pt |
dc.subject.mesh | Liver Neoplasms | pt |
dc.title | Glycopolymers Mediate Suicide Gene Therapy in ASGPR-Expressing Hepatocellular Carcinoma Cells in Tandem with Docetaxel | pt |
dc.type | article | - |
degois.publication.firstPage | 1274 | pt |
degois.publication.lastPage | 1286 | pt |
degois.publication.issue | 3 | pt |
degois.publication.title | Biomacromolecules | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1021/acs.biomac.2c01329 | pt |
degois.publication.volume | 24 | pt |
dc.date.embargo | 2023-03-13 | * |
uc.date.periodoEmbargo | 0 | pt |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.grantfulltext | open | - |
item.openairetype | article | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.cerifentitytype | Publications | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.researchunit | CEMMPRE - Centre for Mechanical Engineering, Materials and Processes | - |
crisitem.author.orcid | 0000-0001-8664-2757 | - |
crisitem.author.orcid | 0000-0001-9351-1704 | - |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D CEMMPRE - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Glycopolymers-Mediate-Suicide-Gene-Therapy-in-ASGPRExpressing-Hepatocellular-Carcinoma-Cells-in-Tandem-with-DocetaxelBiomacromolecules.pdf | 10.33 MB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License