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Title: DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization
Authors: Corduneanu, O. 
Paquim, A. M. Chiorcea 
Diculescu, V. 
Fiuza, S. M. 
Marques, M. P. M. 
Brett, A. M. Oliveira 
Keywords: Animals; Biogenic Polyamines; Carbon; Cattle; Chelating Agents; DNA; Electrochemistry; Electrodes; Electrophoresis; Glass; Graphite; Ligands; Microscopy, Atomic Force; Models, Molecular; Nucleic Acid Conformation; Organometallic Compounds; Oxidation-Reduction; Palladium; Surface Properties
Issue Date: 2010
Serial title, monograph or event: Analytical Chemistry
Volume: 82
Issue: 4
Abstract: The interaction of double-stranded DNA with two polynuclear Pd(II) chelates with the biogenic polyamines spermidine (Spd) and spermine (Spm), Pd(II)-Spd and Pd(II)-Spm, as well as with the free ligands Spd and Spm, was studied using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite (HOPG) surface, voltammetry at a glassy carbon (GC) electrode, and gel electrophoresis. The AFM and voltammetric results showed that the interaction of Spd and Spm with DNA occurred even for a low concentration of polyamines and caused no oxidative damage to DNA. The Pd(II)-Spd and Pd(II)-Spm complexes were found to induce greater morphological changes in the dsDNA conformation, when compared with their ligands. The interaction was specific, inducing distortion and local denaturation of the B-DNA structure with release of some guanine bases. The DNA strands partially opened give rise to palladium intra- and interstrand cross-links, leading to the formation of DNA adducts and aggregates, particularly in the case of the Pd(II)-Spd complex.
DOI: 10.1021/ac902127d
Rights: openAccess
Appears in Collections:I&D QFM-UC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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