Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/45110
Title: | DNA Interaction with Palladium Chelates of Biogenic Polyamines Using Atomic Force Microscopy and Voltammetric Characterization | Authors: | Corduneanu, O. Paquim, A. M. Chiorcea Diculescu, V. Fiuza, S. M. Marques, M. P. M. Brett, A. M. Oliveira |
Keywords: | Animals; Biogenic Polyamines; Carbon; Cattle; Chelating Agents; DNA; Electrochemistry; Electrodes; Electrophoresis; Glass; Graphite; Ligands; Microscopy, Atomic Force; Models, Molecular; Nucleic Acid Conformation; Organometallic Compounds; Oxidation-Reduction; Palladium; Surface Properties | Issue Date: | 2010 | Serial title, monograph or event: | Analytical Chemistry | Volume: | 82 | Issue: | 4 | Abstract: | The interaction of double-stranded DNA with two polynuclear Pd(II) chelates with the biogenic polyamines spermidine (Spd) and spermine (Spm), Pd(II)-Spd and Pd(II)-Spm, as well as with the free ligands Spd and Spm, was studied using atomic force microscopy (AFM) at a highly oriented pyrolytic graphite (HOPG) surface, voltammetry at a glassy carbon (GC) electrode, and gel electrophoresis. The AFM and voltammetric results showed that the interaction of Spd and Spm with DNA occurred even for a low concentration of polyamines and caused no oxidative damage to DNA. The Pd(II)-Spd and Pd(II)-Spm complexes were found to induce greater morphological changes in the dsDNA conformation, when compared with their ligands. The interaction was specific, inducing distortion and local denaturation of the B-DNA structure with release of some guanine bases. The DNA strands partially opened give rise to palladium intra- and interstrand cross-links, leading to the formation of DNA adducts and aggregates, particularly in the case of the Pd(II)-Spd complex. | URI: | https://hdl.handle.net/10316/45110 | DOI: | 10.1021/ac902127d 10.1021/ac902127d |
Rights: | openAccess |
Appears in Collections: | I&D QFM-UC - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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AnalChem_10_82.pdf | 3.2 MB | Adobe PDF | View/Open |
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