Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27752
Title: Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry
Authors: Freitas, Andreia 
Barbosa, Jorge 
Ramos, Fernando 
Keywords: Antibiotics; Multiclass; Multidetection; UHPLC-MS/MS; Liver; Validation
Issue Date: Nov-2014
Publisher: Elsevier
Citation: FREITAS, Andreia; BARBOSA, Jorge; RAMOS, Fernando - Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry. "Journal of Chromatography B". ISSN 1570-0232. (2014)
Abstract: A multiresidue quantitative screening method covering 39 antibiotics from 7 different families by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. A simple sample treatment method consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA) followed by solid-phase extraction (SPE) with a hydrophilic-lipophilic balanced (HLB) cartridge was developed. The methodology was validated, in accordance with Decision 2002/657/EC, by evaluating the following required parameters: decision limit (CCα), detection capability (CCβ), specificity, repeatability and reproducibility. The precision, in terms of the relative standard deviation, was under 22% for all of the compounds, and the recoveries were between 80% and 110%. The CCα and CCβ were determined according to the maximum residue limit (MRL) or the minimum required performance limit (MRPL), when established.
URI: http://hdl.handle.net/10316/27752
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2014.11.008
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D CEFarmacêuticos - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat 
Multidetection of antibiotics in liver tissue by.pdf448.97 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

6
checked on Jun 25, 2019

WEB OF SCIENCETM
Citations

7
checked on Jun 25, 2019

Page view(s)

178
checked on Jul 16, 2019

Download(s)

95
checked on Jul 16, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.