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https://hdl.handle.net/10316/27752
Title: | Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry | Authors: | Freitas, Andreia Barbosa, Jorge Ramos, Fernando |
Keywords: | Antibiotics; Multiclass; Multidetection; UHPLC-MS/MS; Liver; Validation | Issue Date: | Nov-2014 | Publisher: | Elsevier | Citation: | FREITAS, Andreia; BARBOSA, Jorge; RAMOS, Fernando - Multidetection of antibiotics in liver tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry. "Journal of Chromatography B". ISSN 1570-0232. (2014) | Serial title, monograph or event: | Journal of Chromatography B | Abstract: | A multiresidue quantitative screening method covering 39 antibiotics from 7 different families by Ultra-High-Pressure-Liquid-Chromatography tandem Mass Spectrometry (UHPLC-MS/MS) is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. A simple sample treatment method consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA) followed by solid-phase extraction (SPE) with a hydrophilic-lipophilic balanced (HLB) cartridge was developed. The methodology was validated, in accordance with Decision 2002/657/EC, by evaluating the following required parameters: decision limit (CCα), detection capability (CCβ), specificity, repeatability and reproducibility. The precision, in terms of the relative standard deviation, was under 22% for all of the compounds, and the recoveries were between 80% and 110%. The CCα and CCβ were determined according to the maximum residue limit (MRL) or the minimum required performance limit (MRPL), when established. | URI: | https://hdl.handle.net/10316/27752 | ISSN: | 1570-0232 | DOI: | 10.1016/j.jchromb.2014.11.008 | Rights: | openAccess |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais I&D CEFarmacêuticos - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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