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Title: Transcriptome analysis reveals the high ribosomal inhibitory action of 1,4-naphthoquinone on Meloidogyne luci infective second-stage juveniles
Authors: Cardoso, Joana M. S. 
Esteves, Ivânia 
Egas, Conceição 
Braga, Mara E. M. 
Sousa, Herminio C. de 
Abrantes, Isabel 
Maleita, Carla 
Keywords: bionematicide; Meloidogyne; naphthoquinone; plant-parasitic nematodes; transcriptome
Issue Date: 2023
Publisher: Frontiers Media S.A.
Project: PTDC/ASP-PLA/29392/2017 (POCI-01-145-FEDER- 029392) 
POCI-01-0145-FEDER-022184 (GenomePT) 
UIDB/00102/ 2020 
Instituto do Ambiente, Tecnologia e Vida 
Serial title, monograph or event: Frontiers in Plant Science
Volume: 14
Abstract: The root-knot nematode (RKN) Meloidogyne luci presents a threat to the production of several important crops. This nematode species was added to the European Plant Protection Organization Alert list in 2017. The scarce availability of efficient nematicides to control RKN and the phasing out of nematicides from the market have intensified the search for alternatives, such as phytochemicals with bionematicidal properties. The nematicidal activity of 1,4-naphthoquinone (1,4-NTQ) against M. luci has been demonstrated; however, knowledge of the potential mode(s) of action of this compound is still scarce. In this study, the transcriptome profile of M. luci second-stage juveniles (J2), the infective stage, in response to 1,4-NTQ exposure was determined by RNA-seq to identify genes and pathways that might be involved in 1,4-NTQ's mode(s) of action. Control treatments, consisting of nematodes exposed to Tween® 80 (1,4-NTQ solvent) and to water, were included in the analysis. A large set of differentially expressed genes (DEGs) was found among the three tested conditions, and a high number of downregulated genes were found between 1,4-NTQ treatment and water control, reflecting the inhibitory effect of this compound on M. luci, with a great impact on processes related to translation (ribosome pathway). Several other nematode gene networks and metabolic pathways affected by 1,4-NTQ were also identified, clarifying the possible mode of action of this promising bionematicide.
ISSN: 1664-462X
DOI: 10.3389/fpls.2023.1191923
Rights: openAccess
Appears in Collections:FCTUC Eng.Química - Artigos em Revistas Internacionais
I&D CIEPQPF - Artigos em Revistas Internacionais
FMUC Medicina - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D CFE - Artigos em Revistas Internacionais

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