Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106834
Title: | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment | Authors: | Pereira, Sónia Gonçalves Alarico, Susana Tiago, Igor Reis, Diogo Nunes-Costa, Daniela Cardoso, Olga Maranha, Ana Empadinhas, Nuno |
Keywords: | Nontuberculous mycobacteria (NTM); Mycobacterium mucogenicum; Mycobacterium obuense; Mycobacterium paragordonae; Corynebacterineae; Antimicrobial resistance | Issue Date: | 19-Mar-2019 | Publisher: | Springer Nature | Project: | SFRH/BPD/108299/2015 SFRH/BD/117777/2016 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013 |
Serial title, monograph or event: | BMC Microbiology | Volume: | 19 | Issue: | 1 | Abstract: | Background: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevalence in anthropogenic environments and the associated antimicrobial resistance profiles have been neglected. In this work, we sought to determine minimal inhibitory concentrations of 25 antimicrobials against 5 NTM isolates recovered from a tertiary-care hospital surfaces. Antimicrobial susceptibilities of 5 other Corynebacterineae isolated from the same hospital were also determined for their potential clinical relevance. Results: Our phylogenetic study with each of the NTM isolates confirm they belong to Mycobacterium obuense, Mycobacterium mucogenicum and Mycobacterium paragordonae species, the latter initially misidentified as strains of M. gordonae, a species frequently isolated from patients with NTM disease in Portugal. In contrast to other strains, the M. obuense and M. mucogenicum examined here were resistant to several of the CLSI-recommended drugs, suggestive of multidrug-resistant profiles. Surprisingly, M. obuense was susceptible to vancomycin. Their genomes were sequenced allowing detection of gene erm (erythromycin resistance methylase) in M. obuense, explaining its resistance to clarithromycin. Remarkably, and unlike other strains of the genus, the Corynebacterium isolates were highly resistant to penicillin, ciprofloxacin and linezolid. Conclusions: This study highlights the importance of implementing effective measures to screen, accurately identify and control viable NTM and closely related bacteria in hospital settings. Our report on the occurrence of rare NTM species with antibiotic susceptibility profiles that are distinct from those of the corresponding Type strains, along with unexpected resistance mechanisms detected seem to suggest that resistance may be more common than previously thought and also a potential threat to frail and otherwise vulnerable inpatients. | URI: | https://hdl.handle.net/10316/106834 | ISSN: | 1471-2180 | DOI: | 10.1186/s12866-019-1428-4 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D CFE - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais I&D CERES - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Studies-of-antimicrobial-resistance-in-rare-mycobacteria-from-a-nosocomial-environmentBMC-Microbiology.pdf | 1.34 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License