Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/105386
Title: Highly Porous Composite Scaffolds Endowed with Antibacterial Activity for Multifunctional Grafts in Bone Repair
Authors: Neto, Ana S.
Pereira, Patrícia 
Fonseca, Ana C. 
Dias, Carla 
Almeida, Mariana C. 
Barros, Inês 
Miranda, Catarina O. 
Almeida, Luís P. de 
Morais, Paula V. 
Ferreira, José M. F.
Keywords: cuttlefish bone; biphasic calcium phosphate; polymeric coatings; rifampicin; drug delivery system
Issue Date: 2021
Publisher: MDPI
Project: UID/CTM/50011/2019 
UIDB/00285/2020 
UIBD/00511/2020 
UIDB/04539/2020 
CENTRO- 01-0145-FEDER-000008 
CENTRO-01-0145-FEDER-022095 
SFRH/BD/148877/2019 
Serial title, monograph or event: Polymers
Volume: 13
Issue: 24
Abstract: The present study deals with the development of multifunctional biphasic calcium phosphate (BCP) scaffolds coated with biopolymers—poly("-caprolactone) (PCL) or poly(ester urea) (PEU)—loaded with an antibiotic drug, Rifampicin (RFP). The amounts of RFP incorporated into the PCL and PEU-coated scaffolds were 0.55 0.04 and 0.45 0.02 wt%, respectively. The in vitro drug release profiles in phosphate buffered saline over 6 days were characterized by a burst release within the first 8h, followed by a sustained release. The Korsmeyer–Peppas model showed that RFP release was controlled by polymer-specific non-Fickian diffusion. A faster burst release (67.33 1.48%) was observed for the PCL-coated samples, in comparison to that measured (47.23 0.31%) for the PEU-coated samples. The growth inhibitory activity against Escherichia coli and Staphylococcus aureus was evaluated. Although the RFP-loaded scaffolds were effective in reducing bacterial growth for both strains, their effectiveness depends on the particular bacterial strain, as well as on the type of polymer coating, since it rules the drug release behavior. The low antibacterial activity demonstrated by the BCP-PEU-RFP scaffold against E. coli could be a consequence of the lower amount of RFP that is released from this scaffold, when compared with BCP-PCL-RFP. In vitro studies showed excellent cytocompatibility, adherence, and proliferation of human mesenchymal stem cells on the BCP-PEU-RFP scaffold surface. The fabricated highly porous scaffolds that could act as an antibiotic delivery system have great potential for applications in bone regeneration and tissue engineering, while preventing bacterial infections.
URI: https://hdl.handle.net/10316/105386
ISSN: 2073-4360
DOI: 10.3390/polym13244378
Rights: openAccess
Appears in Collections:I&D CEMMPRE - Artigos em Revistas Internacionais
I&D CNC - Artigos em Revistas Internacionais
I&D CIBB - Artigos em Revistas Internacionais
IIIUC - Artigos em Revistas Internacionais
FFUC- Artigos em Revistas Internacionais

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