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Title: | Highly Porous Composite Scaffolds Endowed with Antibacterial Activity for Multifunctional Grafts in Bone Repair | Authors: | Neto, Ana S. Pereira, Patrícia Fonseca, Ana C. Dias, Carla Almeida, Mariana C. Barros, Inês Miranda, Catarina O. Almeida, Luís P. de Morais, Paula V. Ferreira, José M. F. |
Keywords: | cuttlefish bone; biphasic calcium phosphate; polymeric coatings; rifampicin; drug delivery system | Issue Date: | 2021 | Publisher: | MDPI | Project: | UID/CTM/50011/2019 UIDB/00285/2020 UIBD/00511/2020 UIDB/04539/2020 CENTRO- 01-0145-FEDER-000008 CENTRO-01-0145-FEDER-022095 SFRH/BD/148877/2019 |
Serial title, monograph or event: | Polymers | Volume: | 13 | Issue: | 24 | Abstract: | The present study deals with the development of multifunctional biphasic calcium phosphate (BCP) scaffolds coated with biopolymers—poly("-caprolactone) (PCL) or poly(ester urea) (PEU)—loaded with an antibiotic drug, Rifampicin (RFP). The amounts of RFP incorporated into the PCL and PEU-coated scaffolds were 0.55 0.04 and 0.45 0.02 wt%, respectively. The in vitro drug release profiles in phosphate buffered saline over 6 days were characterized by a burst release within the first 8h, followed by a sustained release. The Korsmeyer–Peppas model showed that RFP release was controlled by polymer-specific non-Fickian diffusion. A faster burst release (67.33 1.48%) was observed for the PCL-coated samples, in comparison to that measured (47.23 0.31%) for the PEU-coated samples. The growth inhibitory activity against Escherichia coli and Staphylococcus aureus was evaluated. Although the RFP-loaded scaffolds were effective in reducing bacterial growth for both strains, their effectiveness depends on the particular bacterial strain, as well as on the type of polymer coating, since it rules the drug release behavior. The low antibacterial activity demonstrated by the BCP-PEU-RFP scaffold against E. coli could be a consequence of the lower amount of RFP that is released from this scaffold, when compared with BCP-PCL-RFP. In vitro studies showed excellent cytocompatibility, adherence, and proliferation of human mesenchymal stem cells on the BCP-PEU-RFP scaffold surface. The fabricated highly porous scaffolds that could act as an antibiotic delivery system have great potential for applications in bone regeneration and tissue engineering, while preventing bacterial infections. | URI: | https://hdl.handle.net/10316/105386 | ISSN: | 2073-4360 | DOI: | 10.3390/polym13244378 | Rights: | openAccess |
Appears in Collections: | I&D CEMMPRE - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais |
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