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https://hdl.handle.net/10316/8088
Título: | Estimating gluconeogenesis by NMR isotopomer distribution analysis of [13 C]bicarbonate and [-1 13C]lactate | Autor: | Alves, Tiago Cardoso Nunes, Patrícia Maria Palmeira, Carlos Marques Jones, John Griffith Carvalho, Rui Albuquerque |
Data: | 2008 | Citação: | NMR in Biomedicine. 21:4 (2008) 337-344 | Resumo: | The gluconeogenic contribution to glucose production in livers isolated from rats fasted for 24 h was determined by 13C-NMR isotopomer distribution analysis of secreted glucose enriched from 99% [13C]bicarbonate (n = 4) and 99% [1-13C]lactate (n = 4). Experiments with 3% 2H2O were also performed, allowing the gluconeogenic contribution to be measured by the relative 2H enrichments at positions 5 and 2 of glucose. From 13C-NMR analyses, the contribution of gluconeogenesis to glucose output was estimated to be 93 ± 3% for [13C]bicarbonate perfusion and 91 ± 3% for [1-13C]lactate perfusion, in good agreement with the 2H-NMR analysis of the gluconeogenic contribution to glucose production (100 ± 1% and 99 ± 1%, respectively) and consistent with the expected negligible contribution from glycogenolysis. These results indicate that 13C-NMR analysis of glucose 13C-isotopomer distribution from either [13C]bicarbonate or [1-13C]lactate precursor provides realistic estimates of the gluconeogenic contribution to hepatic glucose output. Copyright © 2007 John Wiley & Sons, Ltd. | URI: | https://hdl.handle.net/10316/8088 | DOI: | 10.1002/nbm.1195 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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