Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/8088
DC FieldValueLanguage
dc.contributor.authorAlves, Tiago Cardoso-
dc.contributor.authorNunes, Patrícia Maria-
dc.contributor.authorPalmeira, Carlos Marques-
dc.contributor.authorJones, John Griffith-
dc.contributor.authorCarvalho, Rui Albuquerque-
dc.date.accessioned2009-02-09T11:10:43Z-
dc.date.available2009-02-09T11:10:43Z-
dc.date.issued2008en_US
dc.identifier.citationNMR in Biomedicine. 21:4 (2008) 337-344en_US
dc.identifier.urihttps://hdl.handle.net/10316/8088-
dc.description.abstractThe gluconeogenic contribution to glucose production in livers isolated from rats fasted for 24 h was determined by 13C-NMR isotopomer distribution analysis of secreted glucose enriched from 99% [13C]bicarbonate (n = 4) and 99% [1-13C]lactate (n = 4). Experiments with 3% 2H2O were also performed, allowing the gluconeogenic contribution to be measured by the relative 2H enrichments at positions 5 and 2 of glucose. From 13C-NMR analyses, the contribution of gluconeogenesis to glucose output was estimated to be 93 ± 3% for [13C]bicarbonate perfusion and 91 ± 3% for [1-13C]lactate perfusion, in good agreement with the 2H-NMR analysis of the gluconeogenic contribution to glucose production (100 ± 1% and 99 ± 1%, respectively) and consistent with the expected negligible contribution from glycogenolysis. These results indicate that 13C-NMR analysis of glucose 13C-isotopomer distribution from either [13C]bicarbonate or [1-13C]lactate precursor provides realistic estimates of the gluconeogenic contribution to hepatic glucose output. Copyright © 2007 John Wiley & Sons, Ltd.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleEstimating gluconeogenesis by NMR isotopomer distribution analysis of [13 C]bicarbonate and [-1 13C]lactateen_US
dc.typearticleen_US
dc.identifier.doi10.1002/nbm.1195en_US
item.fulltextCom Texto completo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.openairetypearticle-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-2639-7697-
crisitem.author.orcid0000-0002-3745-3885-
crisitem.author.orcid0000-0003-1820-0353-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
obra.pdf216.05 kBAdobe PDFView/Open
Show simple item record

SCOPUSTM   
Citations

3
checked on Nov 11, 2022

WEB OF SCIENCETM
Citations 10

3
checked on May 2, 2023

Page view(s) 20

652
checked on Feb 20, 2024

Download(s) 1

7,726
checked on Feb 20, 2024

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.