Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/17993
Título: | Evaluation of the structure–activity relationship of thrombin with thrombin binding aptamers by voltammetry and atomic force microscopy | Autor: | Diculescu, Victor Constantin Paquim, Ana Maria Chiorcea Eritja, Ramon Brett, Ana Maria Oliveira |
Palavras-chave: | Thrombin; Aptamer; Quadruplex; Guanine; AFM; Electrochemistry | Data: | Jul-2011 | Editora: | Elsevier B.V. | Citação: | DICULESCU, Victor Constantin [et al.] - Evaluation of the structure–activity relationship of thrombin with thrombin binding aptamers by voltammetry and atomic force microscopy. "Journal of Electroanalytical Chemistry". ISSN 1572-6657. 656:1-2 (2011) 159-166 | Título da revista, periódico, livro ou evento: | Journal of Electroanalytical Chemistry | Volume: | 656 | Número: | 1-2 | Resumo: | The structure–activity relationship of the complex between thrombin and thrombin binding aptamers (TBA) was evaluated by differential pulse voltammetry at a glassy carbon electrode and atomic force microscopy at a highly oriented pyrolytic graphite electrode. The effects on the interaction with thrombin of TBA primary and secondary structures as well as of its folding properties in the presence of alkaline metals were investigated. The complex between thrombin and single stranded aptamers involved the coiling of the single stranded molecules around thrombin structure leading to the formation of a robust TBA–thrombin complex that maintains the symmetry and conformation of the thrombin molecule. Monitoring both thrombin and TBA oxidation peaks, showed that the thrombin oxidation peaks occur at more positive potentials after TBA–thrombin complex formation. In the presence of K+ ions, the aptamers fold into quadruplex structures that facilitate the interaction with thrombin molecules. The TBA–thrombin complex adsorbs at the surface with the aptamer quadruplex always in preferential contact with the surface, and the thrombin molecules on top of the aptamer quadruplex structure, thus being less accessible to the electrode surface leading to the occurrence of thrombin oxidation peaks at less positive potentials. | URI: | https://hdl.handle.net/10316/17993 | ISSN: | 1572-6657 | DOI: | 10.1016/j.jelechem.2010.11.037 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Química - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Evaluation of the structure-activity relationship.pdf | 2.01 MB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
24
Visto em 22/abr/2024
Citações WEB OF SCIENCETM
5
25
Visto em 2/abr/2024
Visualizações de página 50
379
Visto em 23/abr/2024
Downloads
291
Visto em 23/abr/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.