Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/14426
Título: | Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells | Autor: | Pedroso, Dora C. S. Tellechea, Ana Moura, Liane Fidalgo-Carvalho, Isabel Duarte, João Carvalho, Eugénia Ferreira, Lino |
Data: | Jan-2011 | Editora: | Public Library of Science | Citação: | PEDROSO, Dora C. S. [et al.] - Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells. "PLoS ONE". ISSN 1932-6203. 6:1 (2011) e16114 | Título da revista, periódico, livro ou evento: | PLoS ONE | Volume: | 6 | Número: | 1 | Resumo: | In this study, we developed a methodology to improve the survival, vascular differentiation and regenerative potential of umbilical cord blood (UCB)-derived hematopoietic stem cells (CD34+ cells), by co-culturing the stem cells in a 3D fibrin gel with CD34+-derived endothelial cells (ECs). ECs differentiated from CD34+ cells appear to have superior angiogenic properties to fully differentiated ECs, such as human umbilical vein endothelial cells (HUVECs). Our results indicate that the pro-survival effect of CD34+-derived ECs on CD34+ cells is mediated, at least in part, by bioactive factors released from ECs. This effect likely involves the secretion of novel cytokines, including interleukin-17 (IL-17) and interleukin-10 (IL-10), and the activation of the ERK 1/2 pathway in CD34+ cells. We also show that the endothelial differentiation of CD34+ cells in co-culture with CD34+-derived ECs is mediated by a combination of soluble and insoluble factors. The regenerative potential of this co-culture system was demonstrated in a chronic wound diabetic animal model. The co-transplantation of CD34+ cells with CD34+-derived ECs improved the wound healing relatively to controls, by decreasing the inflammatory reaction and increasing the neovascularization of the wound. | URI: | https://hdl.handle.net/10316/14426 | ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0016114 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Improved Survival, Vascular Differentiation.pdf | 872.58 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
86
Visto em 1/mai/2023
Citações WEB OF SCIENCETM
1
91
Visto em 2/out/2024
Visualizações de página
418
Visto em 15/out/2024
Downloads
252
Visto em 15/out/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.