Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/14426
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dc.contributor.authorPedroso, Dora C. S.-
dc.contributor.authorTellechea, Ana-
dc.contributor.authorMoura, Liane-
dc.contributor.authorFidalgo-Carvalho, Isabel-
dc.contributor.authorDuarte, João-
dc.contributor.authorCarvalho, Eugénia-
dc.contributor.authorFerreira, Lino-
dc.date.accessioned2011-01-31T09:31:58Z-
dc.date.available2011-01-31T09:31:58Z-
dc.date.issued2011-01-
dc.identifier.citationPEDROSO, Dora C. S. [et al.] - Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells. "PLoS ONE". ISSN 1932-6203. 6:1 (2011) e16114-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10316/14426-
dc.description.abstractIn this study, we developed a methodology to improve the survival, vascular differentiation and regenerative potential of umbilical cord blood (UCB)-derived hematopoietic stem cells (CD34+ cells), by co-culturing the stem cells in a 3D fibrin gel with CD34+-derived endothelial cells (ECs). ECs differentiated from CD34+ cells appear to have superior angiogenic properties to fully differentiated ECs, such as human umbilical vein endothelial cells (HUVECs). Our results indicate that the pro-survival effect of CD34+-derived ECs on CD34+ cells is mediated, at least in part, by bioactive factors released from ECs. This effect likely involves the secretion of novel cytokines, including interleukin-17 (IL-17) and interleukin-10 (IL-10), and the activation of the ERK 1/2 pathway in CD34+ cells. We also show that the endothelial differentiation of CD34+ cells in co-culture with CD34+-derived ECs is mediated by a combination of soluble and insoluble factors. The regenerative potential of this co-culture system was demonstrated in a chronic wound diabetic animal model. The co-transplantation of CD34+ cells with CD34+-derived ECs improved the wound healing relatively to controls, by decreasing the inflammatory reaction and increasing the neovascularization of the wound.por
dc.language.isoengpor
dc.publisherPublic Library of Sciencepor
dc.rightsopenAccesspor
dc.titleImproved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cellspor
dc.typearticlepor
degois.publication.firstPagee16114por
degois.publication.issue1por
degois.publication.titlePLoS ONEpor
dc.peerreviewedYespor
dc.identifier.doi10.1371/journal.pone.0016114-
degois.publication.volume6por
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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