Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/14426
DC Field | Value | Language |
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dc.contributor.author | Pedroso, Dora C. S. | - |
dc.contributor.author | Tellechea, Ana | - |
dc.contributor.author | Moura, Liane | - |
dc.contributor.author | Fidalgo-Carvalho, Isabel | - |
dc.contributor.author | Duarte, João | - |
dc.contributor.author | Carvalho, Eugénia | - |
dc.contributor.author | Ferreira, Lino | - |
dc.date.accessioned | 2011-01-31T09:31:58Z | - |
dc.date.available | 2011-01-31T09:31:58Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | PEDROSO, Dora C. S. [et al.] - Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells. "PLoS ONE". ISSN 1932-6203. 6:1 (2011) e16114 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10316/14426 | - |
dc.description.abstract | In this study, we developed a methodology to improve the survival, vascular differentiation and regenerative potential of umbilical cord blood (UCB)-derived hematopoietic stem cells (CD34+ cells), by co-culturing the stem cells in a 3D fibrin gel with CD34+-derived endothelial cells (ECs). ECs differentiated from CD34+ cells appear to have superior angiogenic properties to fully differentiated ECs, such as human umbilical vein endothelial cells (HUVECs). Our results indicate that the pro-survival effect of CD34+-derived ECs on CD34+ cells is mediated, at least in part, by bioactive factors released from ECs. This effect likely involves the secretion of novel cytokines, including interleukin-17 (IL-17) and interleukin-10 (IL-10), and the activation of the ERK 1/2 pathway in CD34+ cells. We also show that the endothelial differentiation of CD34+ cells in co-culture with CD34+-derived ECs is mediated by a combination of soluble and insoluble factors. The regenerative potential of this co-culture system was demonstrated in a chronic wound diabetic animal model. The co-transplantation of CD34+ cells with CD34+-derived ECs improved the wound healing relatively to controls, by decreasing the inflammatory reaction and increasing the neovascularization of the wound. | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.rights | openAccess | por |
dc.title | Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells | por |
dc.type | article | por |
degois.publication.firstPage | e16114 | por |
degois.publication.issue | 1 | por |
degois.publication.title | PLoS ONE | por |
dc.peerreviewed | Yes | por |
dc.identifier.doi | 10.1371/journal.pone.0016114 | - |
degois.publication.volume | 6 | por |
item.fulltext | Com Texto completo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-8525-9967 | - |
crisitem.author.orcid | 0000-0001-8985-9302 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Improved Survival, Vascular Differentiation.pdf | 872.58 kB | Adobe PDF | View/Open |
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