Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/14426
Title: Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells
Authors: Pedroso, Dora C. S. 
Tellechea, Ana 
Moura, Liane 
Fidalgo-Carvalho, Isabel 
Duarte, João 
Carvalho, Eugénia 
Ferreira, Lino 
Issue Date: Jan-2011
Publisher: Public Library of Science
Citation: PEDROSO, Dora C. S. [et al.] - Improved Survival, Vascular Differentiation and Wound Healing Potential of Stem Cells Co-Cultured with Endothelial Cells. "PLoS ONE". ISSN 1932-6203. 6:1 (2011) e16114
Serial title, monograph or event: PLoS ONE
Volume: 6
Issue: 1
Abstract: In this study, we developed a methodology to improve the survival, vascular differentiation and regenerative potential of umbilical cord blood (UCB)-derived hematopoietic stem cells (CD34+ cells), by co-culturing the stem cells in a 3D fibrin gel with CD34+-derived endothelial cells (ECs). ECs differentiated from CD34+ cells appear to have superior angiogenic properties to fully differentiated ECs, such as human umbilical vein endothelial cells (HUVECs). Our results indicate that the pro-survival effect of CD34+-derived ECs on CD34+ cells is mediated, at least in part, by bioactive factors released from ECs. This effect likely involves the secretion of novel cytokines, including interleukin-17 (IL-17) and interleukin-10 (IL-10), and the activation of the ERK 1/2 pathway in CD34+ cells. We also show that the endothelial differentiation of CD34+ cells in co-culture with CD34+-derived ECs is mediated by a combination of soluble and insoluble factors. The regenerative potential of this co-culture system was demonstrated in a chronic wound diabetic animal model. The co-transplantation of CD34+ cells with CD34+-derived ECs improved the wound healing relatively to controls, by decreasing the inflammatory reaction and increasing the neovascularization of the wound.
URI: http://hdl.handle.net/10316/14426
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0016114
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Improved Survival, Vascular Differentiation.pdf872.58 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

54
checked on Nov 8, 2019

WEB OF SCIENCETM
Citations

53
checked on Nov 7, 2019

Page view(s)

148
checked on Dec 4, 2019

Download(s)

63
checked on Dec 4, 2019

Google ScholarTM

Check

Altmetric

Dimensions


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.