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https://hdl.handle.net/10316/114068
Title: | Mitochondrial and Redox Changes in Periodontitis and Type 2 Diabetes Human Blood Mononuclear Cells | Authors: | Ferreira, Ildete L. Costa, Solange Moraes, Bruno Costa, Ana Fokt, Olga Marinho, Daniela Alves, Vera Baptista, Isabel P. Rego, A. Cristina |
Keywords: | periodontitis; type 2 diabetes mellitus; oxidative stress; mitochondria; glutathione; peripheral blood mononuclear cells | Issue Date: | 18-Jan-2023 | Publisher: | MDPI | Project: | UIDB/04539/2020 info:eu-repo/grantAgreement/UIDP/04539/2020 LA/P/0058/2020 |
Serial title, monograph or event: | Antioxidants | Volume: | 12 | Issue: | 2 | Abstract: | Periodontitis (PDT) and type 2 diabetes (T2D) have demonstrated a bidirectional relationship and imbalanced oxidative stress linked to mitochondrial dysfunction. Therefore, we investigated mitochondrial and redox (de)regulation in peripheral blood mononuclear cells (PBMCs) in comorbid T2D-PDT, compared to PDT, T2D patients, and control individuals. PBMCs were analyzed for mitochondrial respiration, reactive oxygen species, antioxidant proteins, and expression of Nrf2-target genes. PDT and T2D-PDT patients exhibited altered periodontal clinical markers, while T2D and T2D-PDT patients displayed increased blood HbA1c. Decreased oxygen consumption and ATP production were observed in the PDT patient's PBMCs. PDT and T2D-PDT PBMCs also evidenced increased H2O2 levels and reduced catalase levels (also detected in T2D patients), whereas a compromised glutathione cycle was observed in T2D-PDT patients. PBMCs from both T2D or T2D-PDT patients showed increased Nrf2 protein levels, enhanced GCL activity and GCL-catalytic subunit protein levels, and maintained GCLc, GST, and HO-1 mRNA levels. In contrast, the expressions of Nrf2-target genes were significantly diminished in the PDT patient's PBMCs. Decreased SOD1 and GST mRNA levels were also observed in CD3+CD8+-lymphocytes derived from PDT and T2D-PDT patients. In conclusion, PBMCs from T2D-PDT patients showed major redox changes, while mononuclear cells from PDT patients showed mitochondrial deregulation and reduced expression of Nrf2-target genes. | URI: | https://hdl.handle.net/10316/114068 | ISSN: | 2076-3921 | DOI: | 10.3390/antiox12020226 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
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Mitochondrial-and-Redox-Changes-in-Periodontitis-and-Type-2-Diabetes-Human-Blood-Mononuclear-CellsAntioxidants.pdf | 1.72 MB | Adobe PDF | View/Open |
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