Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114068
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ferreira, Ildete L. | - |
dc.contributor.author | Costa, Solange | - |
dc.contributor.author | Moraes, Bruno | - |
dc.contributor.author | Costa, Ana | - |
dc.contributor.author | Fokt, Olga | - |
dc.contributor.author | Marinho, Daniela | - |
dc.contributor.author | Alves, Vera | - |
dc.contributor.author | Baptista, Isabel P. | - |
dc.contributor.author | Rego, A. Cristina | - |
dc.date.accessioned | 2024-03-18T10:10:31Z | - |
dc.date.available | 2024-03-18T10:10:31Z | - |
dc.date.issued | 2023-01-18 | - |
dc.identifier.issn | 2076-3921 | - |
dc.identifier.uri | https://hdl.handle.net/10316/114068 | - |
dc.description.abstract | Periodontitis (PDT) and type 2 diabetes (T2D) have demonstrated a bidirectional relationship and imbalanced oxidative stress linked to mitochondrial dysfunction. Therefore, we investigated mitochondrial and redox (de)regulation in peripheral blood mononuclear cells (PBMCs) in comorbid T2D-PDT, compared to PDT, T2D patients, and control individuals. PBMCs were analyzed for mitochondrial respiration, reactive oxygen species, antioxidant proteins, and expression of Nrf2-target genes. PDT and T2D-PDT patients exhibited altered periodontal clinical markers, while T2D and T2D-PDT patients displayed increased blood HbA1c. Decreased oxygen consumption and ATP production were observed in the PDT patient's PBMCs. PDT and T2D-PDT PBMCs also evidenced increased H2O2 levels and reduced catalase levels (also detected in T2D patients), whereas a compromised glutathione cycle was observed in T2D-PDT patients. PBMCs from both T2D or T2D-PDT patients showed increased Nrf2 protein levels, enhanced GCL activity and GCL-catalytic subunit protein levels, and maintained GCLc, GST, and HO-1 mRNA levels. In contrast, the expressions of Nrf2-target genes were significantly diminished in the PDT patient's PBMCs. Decreased SOD1 and GST mRNA levels were also observed in CD3+CD8+-lymphocytes derived from PDT and T2D-PDT patients. In conclusion, PBMCs from T2D-PDT patients showed major redox changes, while mononuclear cells from PDT patients showed mitochondrial deregulation and reduced expression of Nrf2-target genes. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDP/04539/2020 | pt |
dc.relation | LA/P/0058/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | periodontitis | pt |
dc.subject | type 2 diabetes mellitus | pt |
dc.subject | oxidative stress | pt |
dc.subject | mitochondria | pt |
dc.subject | glutathione | pt |
dc.subject | peripheral blood mononuclear cells | pt |
dc.title | Mitochondrial and Redox Changes in Periodontitis and Type 2 Diabetes Human Blood Mononuclear Cells | pt |
dc.type | article | pt |
degois.publication.firstPage | 226 | pt |
degois.publication.issue | 2 | pt |
degois.publication.title | Antioxidants | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/antiox12020226 | - |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2023-01-18 | * |
dc.identifier.pmid | 36829785 | - |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - Associate Laboratory | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0001-6552-4479 | - |
crisitem.author.orcid | 0000-0002-3554-8493 | - |
crisitem.author.orcid | 0000-0003-4503-5365 | - |
crisitem.author.orcid | 0000-0003-0700-3776 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais IIIUC - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Mitochondrial-and-Redox-Changes-in-Periodontitis-and-Type-2-Diabetes-Human-Blood-Mononuclear-CellsAntioxidants.pdf | 1.72 MB | Adobe PDF | View/Open |
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