Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/113613
DC Field | Value | Language |
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dc.contributor.author | Fernandes, Daniel | - |
dc.contributor.author | Sirak, Kendra A. | - |
dc.contributor.author | Cheronet, Olivia | - |
dc.contributor.author | Novak, Mario | - |
dc.contributor.author | Brück, Florian | - |
dc.contributor.author | Zelger, Evelyn | - |
dc.contributor.author | Llanos-Lizcano, Alejandro | - |
dc.contributor.author | Wagner, Anna | - |
dc.contributor.author | Zettl, Anna | - |
dc.contributor.author | Mandl, Kirsten | - |
dc.contributor.author | Duffet Carlson, Kellie Sara | - |
dc.contributor.author | Oberreiter, Victoria | - |
dc.contributor.author | Özdoğan, Kadir T. | - |
dc.contributor.author | Sawyer, Susanna | - |
dc.contributor.author | La Pastina, Francesco | - |
dc.contributor.author | Borgia, Emanuela | - |
dc.contributor.author | Coppa, Alfredo | - |
dc.contributor.author | Dobeš, Miroslav | - |
dc.contributor.author | Velemínský, Petr | - |
dc.contributor.author | Reich, David | - |
dc.contributor.author | Bell, Lynne S. | - |
dc.contributor.author | Pinhasi, Ron | - |
dc.date.accessioned | 2024-02-23T09:59:51Z | - |
dc.date.available | 2024-02-23T09:59:51Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 1088-9051 | pt |
dc.identifier.issn | 1549-5469 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/113613 | - |
dc.description.abstract | Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 individuals of similar archaeological preservation into eight density intervals (2.15 to 2.45 g/cm3, in 0.05 increments). We found that the 2.30 to 2.35 g/cm3 and 2.35 to 2.40 g/cm3 intervals yielded up to 5.28-fold more endogenous unique DNA than the corresponding standard extraction (and up to 8.53-fold before duplicate read removal), while maintaining signals of ancient DNA authenticity and not reducing library complexity. Although small 0.05 g/cm3 intervals may maximally optimize yields, a single separation to remove materials with a density above 2.40 g/cm3 yielded up to 2.57-fold more endogenous DNA on average, which enables the simultaneous separation of samples that vary in preservation or in the type of material analyzed. While requiring no new ancient DNA laboratory equipment and fewer than 30 min of extra laboratory work, the implementation of density separation before DNA extraction can substantially boost endogenous DNA yields without decreasing library complexity. Although subsequent studies are required, we present theoretical and practical foundations that may prove useful when applied to other ancient DNA substrates such as teeth, other bones, and sediments. | pt |
dc.language.iso | eng | pt |
dc.publisher | Cold Spring Harbor Laboratory Press | pt |
dc.relation | Funding was provided by the University of Vienna Research Platform: Mineralogical Preservation of the Human Biome from the Depth of Time (MINERVA). S.S. was funded by the Austrian Science Fund (FWF) M3108-G. This work was also partially supported by a Young Investigator Award to D.M.F. from the Faculty of Life Sciences of the University of Vienna. The excavation of Castel Sozzio was funded by the Grandi Scavi program of the Sapienza University of Rome. | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Powders | pt |
dc.subject.mesh | Plastics | pt |
dc.subject.mesh | DNA | pt |
dc.subject.mesh | DNA, Ancient | pt |
dc.subject.mesh | Petrous Bone | pt |
dc.title | Density separation of petrous bone powders for optimized ancient DNA yields | pt |
dc.type | article | - |
degois.publication.firstPage | 622 | pt |
degois.publication.lastPage | 631 | pt |
degois.publication.issue | 4 | pt |
degois.publication.title | Genome Research | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1101/gr.277714.123 | pt |
degois.publication.volume | 33 | pt |
dc.date.embargo | 2023-04-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | Com Texto completo | - |
crisitem.author.researchunit | CIAS - Research Centre for Anthropology and Health | - |
crisitem.author.orcid | 0000-0002-7434-6552 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CIAS - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Density-separation-of-petrous-bone-powders-for-optimized-ancient-DNA-yieldsGenome-Research.pdf | 899.9 kB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License