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https://hdl.handle.net/10316/110897
Título: | Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets | Autor: | Baldeiras, Inês E. Santos, Rosa M. Rosário, Luís M. |
Palavras-chave: | Cytosolic free Ca2+ concentration; 5-HT amperometry; islet of Langerhans; protein kinase C; pulsatile insulin release; thymeleatoxin | Data: | 2006 | Editora: | Springer Nature | Projeto: | FCT | Título da revista, periódico, livro ou evento: | Biological Research | Volume: | 39 | Número: | 3 | Resumo: | Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 microM) enhanced PIR approximately 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by approximately 40-50%. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion. | URI: | https://hdl.handle.net/10316/110897 | ISSN: | 0716-9760 | DOI: | 10.4067/s0716-97602006000300014 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Protein-kinase-C-isoform-specificity-of-cholinergic-potentiation-of-glucoseinduced-pulsatile-5HTinsulin-release-from-mouse-pancreatic-isletsBiological-Research.pdf | 2 MB | Adobe PDF | Ver/Abrir |
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