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Título: | Modulation of oligodendrocyte differentiation and maturation by combined biochemical and mechanical cues | Autor: | Lourenço, Tânia Paes de Faria, Joana Bippes, Christian A. Maia, João Lopes-da-Silva, José A. Relvas, João B. Grãos, Mário |
Data: | 16-Fev-2016 | Editora: | Springer Nature | Projeto: | This work was funded by the ERDF through Programa Operacional Factores de Competitividade - COMPETE and by national funds by FCT – Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) through grants FCOMP- 01-0124-FEDER-021150 - PTDC/SAU-ENB/119292/2010 attributed to M.G., which included a research fellowship awarded to T.L., and COMPETE funding (Project “Stem cell based platforms for Regenerative and Therapeutic Medicine”, Centro-07-ST24-FEDER-002008). J.B.R acknowledges FCT for grant PTDC/SAUNMC/ 119937/2010 - FCOMP-01-0124-FEDER-021333 and FCT financially supported J.P.F. through fellowship SFRH/BPD/34834/2007. Thanks are also due to FCT/MEC for the financial support to the QOPNA research Unit (FCT UID/QUI/00062/2013), (also through national funds, co-financed by FEDER within the PT2020 Partnership Agreement). | Título da revista, periódico, livro ou evento: | Scientific Reports | Volume: | 6 | Número: | 1 | Resumo: | Extracellular matrix (ECM) proteins play a key role during oligodendrogenesis. While fibronectin (FN) is involved in the maintenance and proliferation of oligodendrocyte progenitor cells (OPCs), merosin (MN) promotes differentiation into oligodendrocytes (OLs). Mechanical properties of the ECM also seem to affect OL differentiation, hence this study aimed to clarify the impact of combined biophysical and biochemical elements during oligodendrocyte differentiation and maturation using synthetic elastic polymeric ECM-like substrates. CG-4 cells presented OPC- or OL-like morphology in response to brain-compliant substrates functionalised with FN or MN, respectively. The expression of the differentiation and maturation markers myelin basic protein--MBP--and proteolipid protein--PLP--(respectively) by primary rat oligodendrocytes was enhanced in presence of MN, but only on brain-compliant conditions, considering the distribution (MBP) or amount (PLP) of the protein. It was also observed that maturation of OLs was attained earlier (by assessing PLP expression) by cells differentiated on MN-functionalised brain-compliant substrates than on standard culture conditions. Moreover, the combination of MN and substrate compliance enhanced the maturation and morphological complexity of OLs. Considering the distinct degrees of stiffness tested ranging within those of the central nervous system, our results indicate that 6.5 kPa is the most suitable rigidity for oligodendrocyte differentiation. | URI: | https://hdl.handle.net/10316/108901 | ISSN: | 2045-2322 | DOI: | 10.1038/srep21563 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Eng.Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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Modulation-of-oligodendrocyte-differentiation-and-maturation-by-combined-biochemical-and-mechanical-cuesScientific-Reports.pdf | 4.13 MB | Adobe PDF | Ver/Abrir |
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