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https://hdl.handle.net/10316/108722
Title: | Synthetic mixed-signal computation in living cells | Authors: | Rubens, Jacob R. Selvaggio, Gianluca Lu, Timothy K. |
Issue Date: | 3-Jun-2016 | Publisher: | Springer Nature | Project: | NSF Graduate Research Fellowship SFRH/BD/51576/2011 National Science Foundation (#1350625 and #1124247) Office of Naval Research (#N000141310424) NIH New Innovator Award (#1DP2OD008435) NIH National Centers for Systems Biology (#1P50GM098792) |
Serial title, monograph or event: | Nature Communications | Volume: | 7 | Issue: | 1 | Abstract: | Living cells implement complex computations on the continuous environmental signals that they encounter. These computations involve both analogue- and digital-like processing of signals to give rise to complex developmental programs, context-dependent behaviours and homeostatic activities. In contrast to natural biological systems, synthetic biological systems have largely focused on either digital or analogue computation separately. Here we integrate analogue and digital computation to implement complex hybrid synthetic genetic programs in living cells. We present a framework for building comparator gene circuits to digitize analogue inputs based on different thresholds. We then demonstrate that comparators can be predictably composed together to build band-pass filters, ternary logic systems and multi-level analogue-to-digital converters. In addition, we interface these analogue-to-digital circuits with other digital gene circuits to enable concentration-dependent logic. We expect that this hybrid computational paradigm will enable new industrial, diagnostic and therapeutic applications with engineered cells. | URI: | https://hdl.handle.net/10316/108722 | ISSN: | 2041-1723 | DOI: | 10.1038/ncomms11658 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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