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Title: Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages
Authors: Ventura, Célia 
Pinto, Fátima V. 
Lourenço, Ana Filipa
Pedrosa, Jorge F. S. 
Fernandes, Susete N.
da Rosa, Rafaela R.
Godinho, Maria Helena
Ferreira, Paulo J. T. 
Louro, Henriqueta
Silva, Maria João Aleixo da 
Keywords: nanofibrillated cellulose; nanocrystalline cellulose; safety assessment; biocompatibility; respiratory effects
Issue Date: 2023
Project: info:eu-repo/grantAgreement/ ToxApp4NanoCELFI/| PTDC/SAU-PUB/32587/2017 
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP/50025/2020/PT/Institute of Nanostructures, Nanomodelling and Nanofabrication 
CA21159—Understanding interaction light-biological surfaces: possibility for new electronic materials and devices (PhoBioS) 
Inpactus Project—innovative products and technologies from eucalyptus/21874/2020 
Volume: 10
Issue: 8
Abstract: Cellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential.
ISSN: 2306-5354
DOI: 10.3390/bioengineering10080986
Rights: openAccess
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais
FCTUC Eng.Química - Artigos em Revistas Internacionais

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