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Title: | Electrochemical and AFM Characterization of G-Quadruplex Electrochemical Biosensors and Applications | Authors: | Chiorcea-Paquim, Ana Maria Eritja, Ramon Oliveira-Brett, Ana Maria |
Issue Date: | 2018 | Publisher: | Hindawi | Project: | SFRH/BPD/92726/2013 UID/EMS/00285/2013 Innovec’EAU (SOE1/P1/F0173) |
Serial title, monograph or event: | Journal of Nucleic Acids | Volume: | 2018 | Abstract: | Guanine-rich DNA sequences are able to form G-quadruplexes, being involved in important biological processes and representing smart self-assembling nanomaterials that are increasingly used in DNA nanotechnology and biosensor technology. G-quadruplex electrochemical biosensors have received particular attention, since the electrochemical response is particularly sensitive to the DNA structural changes from single-stranded, double-stranded, or hairpin into a G-quadruplex configuration. Furthermore, the development of an increased number of G-quadruplex aptamers that combine the G-quadruplex stiffness and self-assembling versatility with the aptamer high specificity of binding to a variety of molecular targets allowed the construction of biosensors with increased selectivity and sensitivity. This review discusses the recent advances on the electrochemical characterization, design, and applications of G-quadruplex electrochemical biosensors in the evaluation of metal ions, G-quadruplex ligands, and other small organic molecules, proteins, and cells. The electrochemical and atomic force microscopy characterization of G-quadruplexes is presented. The incubation time and cations concentration dependence in controlling the G-quadruplex folding, stability, and nanostructures formation at carbon electrodes are discussed. Different G-quadruplex electrochemical biosensors design strategies, based on the DNA folding into a G-quadruplex, the use of G-quadruplex aptamers, or the use of hemin/G-quadruplex DNAzymes, are revisited. | URI: | https://hdl.handle.net/10316/107758 | ISSN: | 2090-0201 | DOI: | 10.1155/2018/5307106 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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Electrochemical-and-AFM-characterization-of-Gquadruplex-electrochemical-biosensors-and-applicationsJournal-of-Nucleic-Acids.pdf | 7.44 MB | Adobe PDF | View/Open |
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