Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/107157
Title: Multi-Organ NMR Metabolomics to Assess In Vivo Overall Metabolic Impact of Cisplatin in Mice
Authors: Carneiro, Tatiana J. 
Araújo, Rita 
Vojtek, Martin
Gonçalves-Monteiro, Salomé
Diniz, Carmen 
Batista de Carvalho, Ana L. M. 
Marques, Maria Paula 
Gil, Ana M
Keywords: cisplatin; mice; mouse model; in vivo; NMR; metabolomics; metabonomics; kidney; liver; breast tissue
Issue Date: 13-Nov-2019
Publisher: MDPI
Project: UID/CTM/50011/2019 
UID/QUI/50006/2019 
UID/MULTI/ 00070/2019 
POCI-01-0145-FEDER-0016786 
Centro-01-0145-FEDER-029956 
PD/BD/135460/2017 
PTDC/QEQ-MED/1890/2014 
SFRH/BD/145920/2019 
Serial title, monograph or event: Metabolites
Volume: 9
Issue: 11
Abstract: This work describes, to our knowledge, the first NMR metabolomics analysis of mice kidney, liver, and breast tissue in response to cisplatin exposure, in search of early metabolic signatures of cisplatin biotoxicity. Balb/c mice were exposed to a single 3.5 mg/kg dose of cisplatin and then euthanized; organs (kidney, liver, breast tissue) were collected at 1, 12, and 48 h. Polar tissue extracts were analyzed by NMR spectroscopy, and the resulting spectra were studied by multivariate and univariate analyses. The results enabled the identification of the most significant deviant metabolite levels at each time point, and for each tissue type, and showed that the largest metabolic impact occurs for kidney, as early as 1 h post-injection. Kidney tissue showed a marked depletion in several amino acids, comprised in an overall 13-metabolites signature. The highest number of changes in all tissues was noted at 12 h, although many of those recovered to control levels at 48 h, with the exception of some persistently deviant tissue-specific metabolites, thus enabling the identification of relatively longer-term effects of cDDP. This work reports, for the first time, early (1-48 h) concomitant effects of cDDP in kidney, liver, and breast tissue metabolism, thus contributing to the understanding of multi-organ cDDP biotoxicity.
URI: https://hdl.handle.net/10316/107157
ISSN: 2218-1989
DOI: 10.3390/metabo9110279
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
I&D QFM-UC - Artigos em Revistas Internacionais

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