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Title: | Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer | Authors: | Martins, Gabriela V. Marques, Ana C. Fortunato, Elvira Sales, M. Goreti F. |
Keywords: | 3-nitrotyrosine; Molecular imprinted polymer; Electrochemical (bio)sensor; Urine biomarker; Carbon-printed electrode | Issue Date: | 2020 | Publisher: | Elsevier | Project: | PTDC/AAG-TEC/5400/2014 & POCI-01-0145- FEDER-016637 NORTE-01-0145-FEDER-024358 UID/CTM/50025/ 2019 SFRH/BD/94159/2013 SFRH/BD/115173/2016 MindGAP/FET-Open/GA829040 |
Serial title, monograph or event: | Sensing and Bio-Sensing Research | Volume: | 28 | Abstract: | Over the last years, paper technology has been widely spread as a more affordable, sustainable and reliable support material to be incorporated in the design of point-of-care (POC) diagnostic devices. However, the single work employing a paper-based device for 3-nitrotyrosine (3-NT), a relevant biomarker for oxidative stress (OS) that is a major origin for many diseases, is incapable of reading successfully complex samples because every species that oxidizes before ~0.75 V will also contribute to the final response. Thus, the introduction of a selective element was made into this set-up by including a molecularly-imprinted polymer (MIP) tailored in-situ. Herein, a novel MIP for 3-NT was assembled directly on a paper platform, made conductive with carbon ink and suitable for an electrochemical transduction. The biomimetic material was produced by electropolymerization of phenol after optimizing several experimental parameters, such a scan-rate, number of cycles, range of potential applied, monomer and template concentrations. Under optimal conditions, the label-free sensor was able to respond to 3-NT from 500 nM to 1 mM, yielding a limit of detection of 22.3 nM. Finally, the applicability of the (bio)sensor was tested by performing calibration assays in human urine samples and a good performance was obtained in terms of sensitivity, selectivity and reproducibility. Overall, the attributes of the herein described sensing approach can be compared to a very limited number of other electrochemical devices, that are still using a conventional three electrode system, making this papersustained device the first electrochemical (bio)sensor with potential to become a portable and low-cost diagnostic tool for 3-NT. In general, the incorporation of molecular imprinting technology coupled to electrochemical transduction enabled the fabrication of suitable smart sensors for wide screening approaches. | URI: | https://hdl.handle.net/10316/106719 | ISSN: | 22141804 | DOI: | 10.1016/j.sbsr.2020.100333 | Rights: | openAccess |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais |
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