Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106532
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Pereira, Patrícia | - |
dc.contributor.author | Barreira, Maria | - |
dc.contributor.author | Cruz, Carla | - |
dc.contributor.author | Tomás, Joana | - |
dc.contributor.author | Luís, Ângelo | - |
dc.contributor.author | Pedro, Augusto Q. | - |
dc.contributor.author | Queiroz, João A. | - |
dc.contributor.author | Sousa, Fani | - |
dc.date.accessioned | 2023-04-06T11:12:07Z | - |
dc.date.available | 2023-04-06T11:12:07Z | - |
dc.date.issued | 2020-10-15 | - |
dc.identifier.issn | 1424-8247 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106532 | - |
dc.description.abstract | The efficacy of brain therapeutics is largely hampered by the presence of the blood-brain barrier (BBB), mainly due to the failure of most (bio) pharmaceuticals to cross it. Accordingly, this study aims to develop nanocarriers for targeted delivery of recombinant precursor microRNA (pre-miR-29b), foreseeing a decrease in the expression of the BACE1 protein, with potential implications in Alzheimer's disease (AD) treatment. Stearic acid (SA) and lactoferrin (Lf) were successfully exploited as brain-targeting ligands to modify cationic polymers (chitosan (CS) or polyethyleneimine (PEI)), and its BBB penetration behavior was evaluated. The intracellular uptake of the dual-targeting drug delivery systems by neuronal cell models, as well as the gene silencing efficiency of recombinant pre-miR-29b, was analyzed in vitro. Labeled pre-miR-29b-CS/PEI-SA-Lf systems showed very strong fluorescence in the cytoplasm and nucleus of RBE4 cells, being verified the delivery of pre-miR-29b to neuronal cells after 1 h transfection. The experiment of transport across the BBB showed that CS-SA-Lf delivered 65% of recombinant pre-miR-29b in a period of 4 h, a significantly higher transport ratio than the 42% found for PEI-SA-Lf in the same time frame. Overall, a novel procedure for the dual targeting of DDS is disclosed, opening new perspectives in nanomedicines delivery, whereby a novel drug delivery system harvests the merits and properties of the different immobilized ligands. | pt |
dc.description.sponsorship | This work was partially supported by the Portuguese Foundation for Science and Technology (FCT), through the project Pest-OE/SAU/UI0709/2014, UIDB/50011/2020 and UIDP/50011/2020, UIDB/00285/2020, and by the project PTDC/BII-BBF/29496/2017 (PUREmiRSILs) funded by FEDER, through the COMPETE2020—Programa Operacional Competitividade e Internacionalização (POCI), and by national funds (OE), through FCT/MCTES. The authors also acknowledge the PPBI-Portuguese Platform of BioImaging through the project POCI-01-0145-FEDER-022122 and Rede Nacional de RMN (PTNMR), supported by FCT-MCTES (ROTEIRO/0031/2013—PINFRA/22161/2016) co-funded by FEDER through COMPETE 2020, POCI, and PORL, and FCT through PIDDAC. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | Pest-OE/SAU/UI0709/2014 | pt |
dc.relation | UIDB/50011/2020 | pt |
dc.relation | UIDP/50011/2020 | pt |
dc.relation | UIDB/50011/2020 | pt |
dc.relation | UIDP/50011/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC/BII-BBF/29496/2017/PT/Ionic Liquid-based supports for pre-miRNAs purification targeting Alzheimer's disease | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9444 - RNIIIE/PINFRA/22161/2016/PT/Portuguese Nuclear Magnetic Resonance Network | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | blood–brain barrier | pt |
dc.subject | chitosan | pt |
dc.subject | drug delivery system | pt |
dc.subject | lactoferrin | pt |
dc.subject | polyethyleneimine | pt |
dc.subject | recombinant miRNA | pt |
dc.title | Brain-Targeted Delivery of Pre-miR-29b Using Lactoferrin-Stearic Acid-Modified-Chitosan/Polyethyleneimine Polyplexes | pt |
dc.type | article | - |
degois.publication.firstPage | 314 | pt |
degois.publication.issue | 10 | pt |
degois.publication.title | Pharmaceuticals | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ph13100314 | pt |
degois.publication.volume | 13 | pt |
dc.date.embargo | 2020-10-15 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.project.grantno | CICECO-Aveiro Institute of Materials | - |
crisitem.author.orcid | 0000-0002-5665-5271 | - |
Appears in Collections: | I&D CEMMPRE - Artigos em Revistas Internacionais FCTUC Eng.Química - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Braintargeted-delivery-of-premir29b-using-lactoferrinstearic-acidmodifiedchitosan-polyethyleneimine-polyplexesPharmaceuticals.pdf | 3.52 MB | Adobe PDF | View/Open |
SCOPUSTM
Citations
17
checked on Aug 5, 2024
WEB OF SCIENCETM
Citations
15
checked on Aug 2, 2024
Page view(s)
101
checked on Aug 13, 2024
Download(s)
46
checked on Aug 13, 2024
Google ScholarTM
Check
Altmetric
Altmetric
This item is licensed under a Creative Commons License