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Title: Multi-Omic Analysis Reveals Different Effects of Sulforaphane on the Microbiome and Metabolome in Old Compared to Young Mice
Authors: Jun, Se-Ran
Cheema, Amrita
Bose, Chhanda
Boerma, Marjan
Palade, Philip T.
Carvalho, Eugenia 
Awasthi, Sanjay
Singh, Sharda P.
Keywords: aging; sulforaphane; gut microbiome; metabolome; biomarkers
Issue Date: 29-Sep-2020
Publisher: MDPI
Serial title, monograph or event: Microorganisms
Volume: 8
Issue: 10
Abstract: Dietary factors modulate interactions between the microbiome, metabolome, and immune system. Sulforaphane (SFN) exerts effects on aging, cancer prevention and reducing insulin resistance. This study investigated effects of SFN on the gut microbiome and metabolome in old mouse model compared with young mice. Young (6-8 weeks) and old (21-22 months) male C57BL/6J mice were provided regular rodent chow ± SFN for 2 months. We collected fecal samples before and after SFN administration and profiled the microbiome and metabolome. Multi-omics datasets were analyzed individually and integrated to investigate the relationship between SFN diet, the gut microbiome, and metabolome. The SFN diet restored the gut microbiome in old mice to mimic that in young mice, enriching bacteria known to be associated with an improved intestinal barrier function and the production of anti-inflammatory compounds. The tricarboxylic acid cycle decreased and amino acid metabolism-related pathways increased. Integration of multi-omic datasets revealed SFN diet-induced metabolite biomarkers in old mice associated principally with the genera, Oscillospira, Ruminococcus, and Allobaculum. Collectively, our results support a hypothesis that SFN diet exerts anti-aging effects in part by influencing the gut microbiome and metabolome. Modulating the gut microbiome by SFN may have the potential to promote healthier aging.
ISSN: 2076-2607
DOI: 10.3390/microorganisms8101500
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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