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https://hdl.handle.net/10316/102837
Título: | Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production | Autor: | Santos, João Campos Marques, Carla Cruz Baptista, Maria Conceição Pimenta, Jorge Teixeira, José Montezinho, Liliana Cagide, Fernando Borges, Fernanda Oliveira, Paulo J. Pereira, Rosa M. L. N. |
Palavras-chave: | sperm; oocytes; mitochondria; mitochondria-targeted dietary antioxidants; ROS; ART | Data: | 22-Mar-2022 | Projeto: | PTDC/BIA-MOL/28607/2017 POCI-01-0145-FEDER-028607 PDR2020-101-03112 UID/QUI/00081/2020 UIDB/04539/2020 UIDP/04539/2020 UIDB/00276/2020 |
Título da revista, periódico, livro ou evento: | Animals | Volume: | 12 | Número: | 7 | Resumo: | Sperm cells are particularly vulnerable to reactive oxygen species (ROS), impairing their fertilizing ability. Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes and capacitated spermatozoa, in concentrations of 0 (control), 1, and 10 µM. After the swim-up, sperm motility (CASA and visual analysis), vitality (eosin-nigrosin), mitochondrial membrane potential (JC1), intracellular ROS, adenosine triphosphate (ATP) levels, and basal metabolism (Seahorse Xfe96) were evaluated. Embryo development and quality were also assessed. Higher cleavage rates were obtained when 1 µM AntiOxBEN2 were added to CAP and FERT media (compared to control, p < 0.04). A positive effect of AntiOxBEN2 on intracellular ROS reduction (p = 0.01), on the increment of mitochondrial membrane potential (p ≤ 0.003) and, consequently, on the sperm quality was identified. However, the highest dose impaired progressive motility, ATP production, and the number of produced embryos. The results demonstrate a beneficial effect of AntiOxBEN2 (1 µM) on sperm capacitation and fertilization processes, thus improving embryonic development. This may constitute a putative novel therapeutic strategy to improve the outcomes of assisted reproductive techniques (ART). | URI: | https://hdl.handle.net/10316/102837 | ISSN: | 2076-2615 | DOI: | 10.3390/ani12070804 | Direitos: | openAccess |
Aparece nas coleções: | I&D CNC - Artigos em Revistas Internacionais |
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Effect-of-a-Novel-Hydroxybenzoic-Acid-Based-Mitochondria-Directed-Antioxidant-Molecule-on-Bovine-Sperm-Function-and-Embryo-ProductionAnimals.pdf | 1.09 MB | Adobe PDF | Ver/Abrir |
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