Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/102837
DC Field | Value | Language |
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dc.contributor.author | Santos, João Campos | - |
dc.contributor.author | Marques, Carla Cruz | - |
dc.contributor.author | Baptista, Maria Conceição | - |
dc.contributor.author | Pimenta, Jorge | - |
dc.contributor.author | Teixeira, José | - |
dc.contributor.author | Montezinho, Liliana | - |
dc.contributor.author | Cagide, Fernando | - |
dc.contributor.author | Borges, Fernanda | - |
dc.contributor.author | Oliveira, Paulo J. | - |
dc.contributor.author | Pereira, Rosa M. L. N. | - |
dc.date.accessioned | 2022-10-17T10:30:34Z | - |
dc.date.available | 2022-10-17T10:30:34Z | - |
dc.date.issued | 2022-03-22 | - |
dc.identifier.issn | 2076-2615 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/102837 | - |
dc.description.abstract | Sperm cells are particularly vulnerable to reactive oxygen species (ROS), impairing their fertilizing ability. Our objective was to study the effect of a novel mitochondrial-directed antioxidant, AntiOxBEN2, on bovine sperm function. This antioxidant was added to the semen capacitation medium (CAP), during the swim-up process, and to the fertilization medium (FERT) during the co-incubation of matured oocytes and capacitated spermatozoa, in concentrations of 0 (control), 1, and 10 µM. After the swim-up, sperm motility (CASA and visual analysis), vitality (eosin-nigrosin), mitochondrial membrane potential (JC1), intracellular ROS, adenosine triphosphate (ATP) levels, and basal metabolism (Seahorse Xfe96) were evaluated. Embryo development and quality were also assessed. Higher cleavage rates were obtained when 1 µM AntiOxBEN2 were added to CAP and FERT media (compared to control, p < 0.04). A positive effect of AntiOxBEN2 on intracellular ROS reduction (p = 0.01), on the increment of mitochondrial membrane potential (p ≤ 0.003) and, consequently, on the sperm quality was identified. However, the highest dose impaired progressive motility, ATP production, and the number of produced embryos. The results demonstrate a beneficial effect of AntiOxBEN2 (1 µM) on sperm capacitation and fertilization processes, thus improving embryonic development. This may constitute a putative novel therapeutic strategy to improve the outcomes of assisted reproductive techniques (ART). | pt |
dc.description.sponsorship | Associação Cognitária Vasco da Gama, Coimbra, Portugal—Curricular Internships (grant ACVG/EC/2020), | pt |
dc.language.iso | eng | pt |
dc.relation | PTDC/BIA-MOL/28607/2017 | pt |
dc.relation | POCI-01-0145-FEDER-028607 | pt |
dc.relation | PDR2020-101-03112 | pt |
dc.relation | UID/QUI/00081/2020 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | UIDP/04539/2020 | pt |
dc.relation | UIDB/00276/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | sperm | pt |
dc.subject | oocytes | pt |
dc.subject | mitochondria | pt |
dc.subject | mitochondria-targeted dietary antioxidants | pt |
dc.subject | ROS | pt |
dc.subject | ART | pt |
dc.title | Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production | pt |
dc.type | article | - |
degois.publication.firstPage | 804 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | Animals | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ani12070804 | pt |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2022-03-22 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Centre for Interdisciplinary Research in Animal Health | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-3796-0238 | - |
crisitem.author.orcid | 0000-0003-0834-5698 | - |
crisitem.author.orcid | 0000-0002-5201-9948 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Effect-of-a-Novel-Hydroxybenzoic-Acid-Based-Mitochondria-Directed-Antioxidant-Molecule-on-Bovine-Sperm-Function-and-Embryo-ProductionAnimals.pdf | 1.09 MB | Adobe PDF | View/Open |
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