Please use this identifier to cite or link to this item:
Title: Monoamine oxidase and semicarbazide-sensitive amine oxidase kinetic analysis in mesenteric arteries of patients with type 2 diabetes
Authors: Nunes, S. F. 
Figueiredo, I. V. 
Pereira, João 
Lemos, E. T. 
Reis, F. 
Teixeira, F. 
Caramona, M. M. 
Keywords: Human mesenteric arteries; Monoamine oxidase; Semicarbazide-sensitive amine oxidase; Type 2 diabetes; Noradrenaline content
Issue Date: 2011
Project: FCT - SFRH/BD/2005 
Serial title, monograph or event: Physiological Research
Volume: 60
Issue: 2
Abstract: Monoamine oxidase (MAO, type A and B) and semicarbazide-sensitive amine oxidase (SSAO) metabolize biogenic amines, however, the impact of these enzymes in arteries from patients with type 2 diabetes remains poorly understood. We investigated the kinetic parameters of the enzymes to establish putative correlations with noradrenaline (NA) content and patient age in human mesenteric arteries from type 2 diabetic patients. The kinetic parameters were evaluated by radiochemical assay and NA content by high-performance liquid chromatography (HPLC). The activity of MAO-A and SSAO in type 2 diabetic vascular tissues was significantly lower compared to the activity obtained in non-diabetic tissues. In the correlation between MAO-A (K(m)) and NA content, we found a positive correlation for both the diabetic and non-diabetic group, but no correlation was established for patient age. In both groups, MAO-B (V(max)) showed a negative correlation with age. The results show that MAO-A and SSAO activities and NA content of type 2 diabetic tissues are lower compared to the non-diabetic tissues, while MAO-B activity remained unchanged. These remarks suggest that MAO-A and SSAO may play an important role in vascular tissue as well as in the vascular pathophysiology of type 2 diabetes.
ISSN: 1802-9973
DOI: 10.33549/physiolres.931982
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais

Files in This Item:
Show full item record


checked on May 20, 2024


checked on May 2, 2024

Page view(s)

checked on May 21, 2024


checked on May 21, 2024

Google ScholarTM




This item is licensed under a Creative Commons License Creative Commons