Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/102755
Title: Monoamine oxidase and semicarbazide-sensitive amine oxidase kinetic analysis in mesenteric arteries of patients with type 2 diabetes
Authors: Nunes, S. F. 
Figueiredo, I. V. 
Pereira, João 
Lemos, E. T. 
Reis, F. 
Teixeira, F. 
Caramona, M. M. 
Keywords: Human mesenteric arteries; Monoamine oxidase; Semicarbazide-sensitive amine oxidase; Type 2 diabetes; Noradrenaline content
Issue Date: 2011
Project: FCT - SFRH/BD/2005 
Serial title, monograph or event: Physiological Research
Volume: 60
Issue: 2
Abstract: Monoamine oxidase (MAO, type A and B) and semicarbazide-sensitive amine oxidase (SSAO) metabolize biogenic amines, however, the impact of these enzymes in arteries from patients with type 2 diabetes remains poorly understood. We investigated the kinetic parameters of the enzymes to establish putative correlations with noradrenaline (NA) content and patient age in human mesenteric arteries from type 2 diabetic patients. The kinetic parameters were evaluated by radiochemical assay and NA content by high-performance liquid chromatography (HPLC). The activity of MAO-A and SSAO in type 2 diabetic vascular tissues was significantly lower compared to the activity obtained in non-diabetic tissues. In the correlation between MAO-A (K(m)) and NA content, we found a positive correlation for both the diabetic and non-diabetic group, but no correlation was established for patient age. In both groups, MAO-B (V(max)) showed a negative correlation with age. The results show that MAO-A and SSAO activities and NA content of type 2 diabetic tissues are lower compared to the non-diabetic tissues, while MAO-B activity remained unchanged. These remarks suggest that MAO-A and SSAO may play an important role in vascular tissue as well as in the vascular pathophysiology of type 2 diabetes.
URI: https://hdl.handle.net/10316/102755
ISSN: 1802-9973
0862-8408
DOI: 10.33549/physiolres.931982
Rights: openAccess
Appears in Collections:FFUC- Artigos em Revistas Internacionais
I&D IBILI - Artigos em Revistas Internacionais

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