Please use this identifier to cite or link to this item:
|Title:||Toxicity Going Nano: Ionic Versus Engineered Cu Nanoparticles Impacts on the Physiological Fitness of the Model Diatom Phaeodactylum tricornutum||Authors:||Franzitta, Marco
Cabrita, Maria Teresa
Matos, Ana Rita
Marques, João Carlos
Goessling, Johannes W.
Santos, Patrick Reis
Fonseca, Vanessa F.
|Keywords:||CuO nanoparticles; photobiology; oxidative stress; lipid metabolism; phytoplankton; cytotoxicity; marine systems||Issue Date:||2020||Project:||PTDC/CTA-AMB/30056/2017 (OPTOX)
investigation contracts (CEECIND/00511/2017 and DL57/2016/CP1479/CT0024)
FCT - postdoctoral grant (SFRH/BPD/95784/2013).
co-funding through the NanoTRAINforGrowth II Program (project 2000032) by the European Commission through the Horizon 2020 Marie Skłodowska-Curie COFUND Program (2015), and by the International Iberian Nanotechnology Laboratory
|Serial title, monograph or event:||Frontiers in Marine Science||Volume:||7||Abstract:||Increasing input of Metal Engineered Nano Particles (MeENPs) in marine ecosystems has raised concerns about their potential toxicity on phytoplankton. Given the lack of knowledge on MeENPs impact on these important primary producers, the effects of Copper Oxide (CuO) ENPs on growth, physiology, pigment profiles, fatty acid (FA) metabolism, and oxidative stress were investigated in the model diatom Pheodactylum tricornutum, to provide suitable biomarkers of CuO ENP exposure versus its ionic counterpart. Diatom growth was inhibited by CuO ENPs but not Ionic Cu, suggesting CuO ENP cytotoxicity. Pulse Modulated Amplitude (PAM) phenotyping evidenced a decrease in the electron transport energy flux, pointing to a reduction in chemical energy generation following CuO ENPs exposure, as well as an increase in the content of the non-functional Cu-substituted chlorophyll a (CuChl a). A significant decrease in eicosapentaenoic acid (C20:5) associated with a significant rise in thylakoid membranes FAs reflected the activation of counteractive measures to photosynthetic impairment. Significant increase in the omega 6/omega 3 ratio, underline expectable negative repercussions to marine food webs. Increased thiobarbituric acid reactive substances reflected heightened oxidative stress by CuO ENP. Enhanced Glutathione Reductase and Ascorbate Peroxidase activity were also more evident for CuO ENPs than ionic Cu. Overall, observed molecular changes highlighted a battery of possible suitable biomarkers to efficiently determine the harmful effects of CuO ENPs. The results suggest that the occurrence and contamination of these new forms of metal contaminants can impose added stress to the marine diatom community, which could have significant impacts on marine ecosystems, namely through a reduction of the primary productivity, oxygen production and omega 6 production, all essential to sustain heterotrophic marine life.||URI:||https://hdl.handle.net/10316/101300||ISSN:||2296-7745||DOI:||10.3389/fmars.2020.539827||Rights:||openAccess|
|Appears in Collections:||I&D MARE - Artigos em Revistas Internacionais|
Show full item record
checked on Nov 17, 2022
checked on Nov 28, 2023
checked on Nov 28, 2023
This item is licensed under a Creative Commons License