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Title: A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results
Authors: Ribeiro, Carolina
Gante, Inês Raquel Cardoso 
Dias, Margarida
Gomes, Ana
Silva, Henriqueta 
Keywords: Biomarkers; Breast cancer; Microenvironment; One-step nucleic acid amplification; Sentinel lymph node
Issue Date: 2021
Project: POCI-01-0145-FEDER-022184/GenomePT-National Laboratory for Genome Sequencing and Analysis 
Serial title, monograph or event: Molecular and Clinical Oncology
Volume: 15
Issue: 4
Abstract: The one-step nucleic acid amplification (OSNA) assay is a molecular method used for detecting breast cancer (BC) metastasis in sentinel lymph nodes (SLNs). However, this method has a major disadvantage, since it prevents tissue structure analysis, while only one molecular marker can be evaluated, namely cytokeratin 19 mRNA. The aim of the present study was to evaluate whether an OSNA-discarded sample could be suitable for the gene expression analysis of the SLN microenvironment. The remaining intermediate phase of the centrifuged SLN homogenate obtained from the OSNA assay of samples from two patients with BC was used for mRNA extraction. Subsequently, the expression of five genes, namely forkhead box, cluster of differentiation 4 and three control genes, was determined by reverse transcription-quanti-tative PCR analysis. The results demonstrated that high-quality RNA was extracted. Therefore, this RNA may be used for gene expression analyses to predict novel molecular biomarkers associated with immuno-inflammatory microenvironment. © 2021, Spandidos Publications. All rights reserved.
ISSN: 2049-9450
DOI: 10.3892/mco.2021.2380
Rights: embargoedAccess
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais

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