Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/95728
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dc.contributor.authorRibeiro, Carolina-
dc.contributor.authorGante, Inês Raquel Cardoso-
dc.contributor.authorDias, Margarida-
dc.contributor.authorGomes, Ana-
dc.contributor.authorSilva, Henriqueta-
dc.date.accessioned2021-09-14T16:08:43Z-
dc.date.available2021-09-14T16:08:43Z-
dc.date.issued2021-
dc.identifier.issn2049-9450pt
dc.identifier.issn2049-9469pt
dc.identifier.urihttps://hdl.handle.net/10316/95728-
dc.description.abstractThe one-step nucleic acid amplification (OSNA) assay is a molecular method used for detecting breast cancer (BC) metastasis in sentinel lymph nodes (SLNs). However, this method has a major disadvantage, since it prevents tissue structure analysis, while only one molecular marker can be evaluated, namely cytokeratin 19 mRNA. The aim of the present study was to evaluate whether an OSNA-discarded sample could be suitable for the gene expression analysis of the SLN microenvironment. The remaining intermediate phase of the centrifuged SLN homogenate obtained from the OSNA assay of samples from two patients with BC was used for mRNA extraction. Subsequently, the expression of five genes, namely forkhead box, cluster of differentiation 4 and three control genes, was determined by reverse transcription-quanti-tative PCR analysis. The results demonstrated that high-quality RNA was extracted. Therefore, this RNA may be used for gene expression analyses to predict novel molecular biomarkers associated with immuno-inflammatory microenvironment. © 2021, Spandidos Publications. All rights reserved.pt
dc.language.isoengpt
dc.relationPOCI-01-0145-FEDER-022184/GenomePT-National Laboratory for Genome Sequencing and Analysispt
dc.relationUIDB/04539/2020pt
dc.rightsembargoedAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt
dc.subjectBiomarkerspt
dc.subjectBreast cancerpt
dc.subjectMicroenvironmentpt
dc.subjectOne-step nucleic acid amplificationpt
dc.subjectSentinel lymph nodept
dc.titleA new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary resultspt
dc.typearticle-
degois.publication.issue4pt
degois.publication.titleMolecular and Clinical Oncologypt
dc.relation.publisherversionhttps://www.spandidos-publications.com/10.3892/mco.2021.2380pt
dc.peerreviewedyespt
dc.identifier.doi10.3892/mco.2021.2380pt
degois.publication.volume15pt
dc.date.embargo2021-06-30*
uc.date.periodoEmbargo180pt
item.fulltextCom Texto completo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextopen-
item.openairetypearticle-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.orcid0000-0003-1589-0416-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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