Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/95728
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Ribeiro, Carolina | - |
dc.contributor.author | Gante, Inês Raquel Cardoso | - |
dc.contributor.author | Dias, Margarida | - |
dc.contributor.author | Gomes, Ana | - |
dc.contributor.author | Silva, Henriqueta | - |
dc.date.accessioned | 2021-09-14T16:08:43Z | - |
dc.date.available | 2021-09-14T16:08:43Z | - |
dc.date.issued | 2021 | - |
dc.identifier.issn | 2049-9450 | pt |
dc.identifier.issn | 2049-9469 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/95728 | - |
dc.description.abstract | The one-step nucleic acid amplification (OSNA) assay is a molecular method used for detecting breast cancer (BC) metastasis in sentinel lymph nodes (SLNs). However, this method has a major disadvantage, since it prevents tissue structure analysis, while only one molecular marker can be evaluated, namely cytokeratin 19 mRNA. The aim of the present study was to evaluate whether an OSNA-discarded sample could be suitable for the gene expression analysis of the SLN microenvironment. The remaining intermediate phase of the centrifuged SLN homogenate obtained from the OSNA assay of samples from two patients with BC was used for mRNA extraction. Subsequently, the expression of five genes, namely forkhead box, cluster of differentiation 4 and three control genes, was determined by reverse transcription-quanti-tative PCR analysis. The results demonstrated that high-quality RNA was extracted. Therefore, this RNA may be used for gene expression analyses to predict novel molecular biomarkers associated with immuno-inflammatory microenvironment. © 2021, Spandidos Publications. All rights reserved. | pt |
dc.language.iso | eng | pt |
dc.relation | POCI-01-0145-FEDER-022184/GenomePT-National Laboratory for Genome Sequencing and Analysis | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.rights | embargoedAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt |
dc.subject | Biomarkers | pt |
dc.subject | Breast cancer | pt |
dc.subject | Microenvironment | pt |
dc.subject | One-step nucleic acid amplification | pt |
dc.subject | Sentinel lymph node | pt |
dc.title | A new application to one‑step nucleic acid amplification‑discarded sample in breast cancer: Preliminary results | pt |
dc.type | article | - |
degois.publication.issue | 4 | pt |
degois.publication.title | Molecular and Clinical Oncology | pt |
dc.relation.publisherversion | https://www.spandidos-publications.com/10.3892/mco.2021.2380 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3892/mco.2021.2380 | pt |
degois.publication.volume | 15 | pt |
dc.date.embargo | 2021-06-30 | * |
uc.date.periodoEmbargo | 180 | pt |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
crisitem.author.orcid | 0000-0003-1589-0416 | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
mco_15_4_2380_PDF.pdf | 369.87 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
1
Visto em 9/nov/2022
Visualizações de página
129
Visto em 24/jul/2024
Downloads
101
Visto em 24/jul/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Este registo está protegido por Licença Creative Commons