Please use this identifier to cite or link to this item: https://hdl.handle.net/10316/88171
Title: A light-triggerable nanoparticle library for the controlled release of non-coding RNAs
Authors: Josephine, Blersch
Vitor, Francisco
Rebelo, Catarina
Adrian, Jimenez-Balsa
Antunes, Helena
Carlo, Gonzato
Pinto, Sandra
Simões, Susana 
Klaus, Liedl
Karsten, Haupt
Ferreira, Lino
Issue Date: 14-Nov-2019
Publisher: Wiley
Serial title, monograph or event: Angewandte Chemie International Edition
Abstract: RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500%) than commercial Lipofectamine in gene knockdown activity. These formulations had differential internalization by skin cells and the endosomal escape was rapid (minutes range) as shown by the recruitment of galectin-8. The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scramble miRNA. Light-activatable NPs offer a new strategy to deliver topically non-coding RNAs.
URI: https://hdl.handle.net/10316/88171
DOI: 10.1002/anie.201911398
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

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