Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8319
Title: Sample sonication after trichloroacetic acid precipitation increases protein recovery from cultured hippocampal neurons, and improves resolution and reproducibility in two-dimensional gel electrophoresis
Authors: Manadas, Bruno J. 
Vougas, Konstantinos 
Fountoulakis, Michael 
Duarte, Carlos B. 
Issue Date: 2006
Citation: ELECTROPHORESIS. 27:9 (2006) 1825-1831
Abstract: Protein precipitation with TCA followed by acetone washing is frequently used to clean samples before 2-DE. However, the difficulty in solubilizing TCA-precipitated proteins causes some variability in 2-D gels and makes it difficult to detect some proteins. In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and improves reproducibility and matching ratios between gels when analyzed by specialized software.
URI: http://hdl.handle.net/10316/8319
DOI: 10.1002/elps.200500757
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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