Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8319
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dc.contributor.authorManadas, Bruno J.-
dc.contributor.authorVougas, Konstantinos-
dc.contributor.authorFountoulakis, Michael-
dc.contributor.authorDuarte, Carlos B.-
dc.date.accessioned2009-02-09T14:33:11Z-
dc.date.available2009-02-09T14:33:11Z-
dc.date.issued2006en_US
dc.identifier.citationELECTROPHORESIS. 27:9 (2006) 1825-1831en_US
dc.identifier.urihttp://hdl.handle.net/10316/8319-
dc.description.abstractProtein precipitation with TCA followed by acetone washing is frequently used to clean samples before 2-DE. However, the difficulty in solubilizing TCA-precipitated proteins causes some variability in 2-D gels and makes it difficult to detect some proteins. In this work we show that sonication of the samples, after TCA precipitation followed by elution in sample buffer, increases total protein recovery, and improves reproducibility and matching ratios between gels when analyzed by specialized software.en_US
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleSample sonication after trichloroacetic acid precipitation increases protein recovery from cultured hippocampal neurons, and improves resolution and reproducibility in two-dimensional gel electrophoresisen_US
dc.typearticleen_US
dc.identifier.doi10.1002/elps.200500757en_US
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0002-1474-0208-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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