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Title: Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: A simple analysis by 13C NMR of urinary menthol glucuronide
Authors: Mendes, Ana C. 
Caldeira, M. Madalena 
Silva, Cláudia 
Burgess, Shawn C. 
Merritt, Matthew E. 
Gomes, Filipe 
Barosa, Cristina 
Delgado, Teresa C. 
Franco, Fátima 
Monteiro, Pedro 
Providência, Luís 
Jones, John G. 
Issue Date: 2006
Citation: Magnetic Resonance in Medicine. 56:5 (2006) 1121-1125
Abstract: Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-13C]glucose. Glucuronide 13C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of 13C-13C spin-coupled multiplet components within each 13C resonance by 13C NMR. The [U-13C]glucuronide isotopomer derived via direct pathway conversion of [U-13C]glucose to [U-13C]UDP-glucose was resolved from [1,2,3-13C3]- and [1,2-13C2]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-13C]glucose. In a second study, a group of four overnight-fasted patients (63 ± 10 kg) with severe heart failure were given peppermint oil and infused with [U-13C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-13C]glucose enrichment of 4.6% ± 0.6%. Menthol glucuronide was harvested and glucuronide 13C-isotopomers were analyzed by 13C NMR. [U-13C]glucuronide enrichment was 0.6% ± 0.1%, and the sum of [1,2,3-13C3] and [1,2-13C2]glucuronide enrichments was 0.9% ± 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% ± 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% ± 10% of GP. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc.
DOI: 10.1002/mrm.21057
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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