Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8238
DC Field | Value | Language |
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dc.contributor.author | Mendes, Ana C. | - |
dc.contributor.author | Caldeira, M. Madalena | - |
dc.contributor.author | Silva, Cláudia | - |
dc.contributor.author | Burgess, Shawn C. | - |
dc.contributor.author | Merritt, Matthew E. | - |
dc.contributor.author | Gomes, Filipe | - |
dc.contributor.author | Barosa, Cristina | - |
dc.contributor.author | Delgado, Teresa C. | - |
dc.contributor.author | Franco, Fátima | - |
dc.contributor.author | Monteiro, Pedro | - |
dc.contributor.author | Providência, Luís | - |
dc.contributor.author | Jones, John G. | - |
dc.date.accessioned | 2009-02-09T14:32:10Z | - |
dc.date.available | 2009-02-09T14:32:10Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Magnetic Resonance in Medicine. 56:5 (2006) 1121-1125 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8238 | - |
dc.description.abstract | Menthol glucuronide was isolated from the urine of a healthy 70-kg female subject following ingestion of 400 mg of peppermint oil and 6 g of 99% [U-13C]glucose. Glucuronide 13C-excess enrichment levels were 4-6% and thus provided high signal-to-noise ratios (SNRs) for confident assignment of 13C-13C spin-coupled multiplet components within each 13C resonance by 13C NMR. The [U-13C]glucuronide isotopomer derived via direct pathway conversion of [U-13C]glucose to [U-13C]UDP-glucose was resolved from [1,2,3-13C3]- and [1,2-13C2]glucuronide isotopomers derived via Cori cycle or indirect pathway metabolism of [U-13C]glucose. In a second study, a group of four overnight-fasted patients (63 ± 10 kg) with severe heart failure were given peppermint oil and infused with [U-13C]glucose for 4 hr (14 mg/kg prime, 0.12 mg/kg/min constant infusion) resulting in a steady-state plasma [U-13C]glucose enrichment of 4.6% ± 0.6%. Menthol glucuronide was harvested and glucuronide 13C-isotopomers were analyzed by 13C NMR. [U-13C]glucuronide enrichment was 0.6% ± 0.1%, and the sum of [1,2,3-13C3] and [1,2-13C2]glucuronide enrichments was 0.9% ± 0.2%. From these data, flux of plasma glucose to hepatic UDPG was estimated to be 15% ± 4% that of endogenous glucose production (EGP), and the Cori cycle accounted for at least 32% ± 10% of GP. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Hepatic UDP-glucose 13C isotopomers from [U-13C]glucose: A simple analysis by 13C NMR of urinary menthol glucuronide | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1002/mrm.21057 | en_US |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.fulltext | Com Texto completo | - |
item.languageiso639-1 | en | - |
crisitem.author.orcid | 0000-0002-6631-207X | - |
crisitem.author.orcid | 0000-0002-3745-3885 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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