Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8200
Title: Exquisite Regioselectivity and Increased Transesterification Activity of an Immobilized Bacillus subtilis Protease
Authors: Ferreira, Lino 
Ramos, M. A. 
Gil, Maria Helena 
Dordick, Jonathan S. 
Issue Date: 2002
Citation: Biotechnology Progress. 18:5 (2002) 986-993
Abstract: Commercially available proteases and lipases were screened for their ability to acylate regioselectively sucrose with divinyladipate either in pyridine or dimethylformamide (DMF). The protease (EC 3.4.21.62) from Bacillus subtilis (Proleather FG-F) exhibited the highest conversion (100% in 24 h of reaction in DMF) yielding sucrose 2- O-vinyladipate as main product. The enzyme preference for a secondary hydroxyl group is a distinct feature of this biocatalyst compared to others described in the literature. Two sets of chemically distinct silica supports were used for Proleather immobilization presenting terminal amino (SAPTES) or hydroxyl groups (STESPM-pHEMA). The percentage of immobilized enzyme was smaller in SAPTES (7-17%) than in STESPM-pHEMA (52-56%), yet Proleather immobilized into SAPTES supports presented higher total and specific hydrolytic activity. The highest total and specific activities were obtained with STESPM-pHEMA and SAPTES, respectively. Silicas with large pore (bimodal distribution of pores, 130/1200 Å, denoted as S1000) presented higher specific activities relative to those with smaller pore sizes. Furthermore, the synthetic specific activity of S1000SAPTES immobilized protease was ca. 10-fold higher than that of the free enzyme. In addition to sucrose, the immobilized protease was used to acylate methyl alpha-d-glucopyranoside, trehalose, and maltose in nearly anhydrous DMF. Finally, immobilized Proleather was reasonably stable, retaining ca. 55% activity after six reaction cycles.
URI: http://hdl.handle.net/10316/8200
DOI: 10.1021/bp0255457
Rights: openAccess
Appears in Collections:FCTUC Eng.Química - Artigos em Revistas Internacionais

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