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https://hdl.handle.net/10316/8189
Title: | Selective extraction of carotenoids from the microalga Dunaliella salina with retention of viability | Authors: | Hejazi, M. A. Lamarliere, C. de Rocha, J. M. S. Vermuë, M. Tramper, J. Wijffels, R. H. |
Issue Date: | 2002 | Citation: | Biotechnology and Bioengineering. 79:1 (2002) 29-36 | Abstract: | Simultaneous production and selective extraction of β-carotene from living cells of in a two-phase system of aqueous and organic phases has been investigated. Solvents with values of log Poctanol, which denotes hydrophobicity of a compound, ranging from 3 to 9 were used as organic phase. Viability and activity of in the presence of organic solvents were checked by microscopic observation and photosynthetic oxygen-production-rate measurements, respectively. Extraction ability of different solvents for both β-carotene and chlorophyll was determined spectrophotometrically. In addition, β-carotene contents of the cells growing in the aqueous phase and extracted β-carotene by the different organic phases were quantified by the same method. Results showed that solvents having log Poctanol > 6 can be considered biocompatible for this alga. Moreover, pigment extraction ability of a solvent is inversely dependent on its log Poctanol value. By increasing the degenerative hydrophobicity the extraction ability for both chlorophyll and β-carotene, decreases. However, this decrease is more profound for chlorophyll. Therefore, selective extraction of β-carotene becomes feasible. Comparison of the total β-carotene produced in the presence and in the absence of solvents shows that the presence of a second phase of biocompatible solvents in the culture media may induce the β-carotene production pathway. The β-carotene productivity per cell in a two-phase system with dodecane was the highest observed. Extraction ability of the biocompatible solvents dodecane, tetradecan, and hexadecane was similar. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 79: 29-36, 2002. | URI: | https://hdl.handle.net/10316/8189 | DOI: | 10.1002/bit.10270 | Rights: | openAccess |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais |
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