Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8138
Title: Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
Authors: Ferreira, A. C. 
Morais, P. V. 
Gomes, C. 
Costa, M. S. da 
Issue Date: 1996
Citation: Journal of Applied Microbiology. 80:5 (1996) 479-486
Abstract: The microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of wholecell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.
URI: http://hdl.handle.net/10316/8138
DOI: 10.1111/j.1365-2672.1996.tb03246.x
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
obra.pdf644.84 kBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

18
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations

17
checked on Feb 18, 2020

Page view(s)

250
checked on Feb 26, 2020

Download(s) 50

455
checked on Feb 26, 2020

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.