Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/7819
Title: Mannosylglycerate is essential for osmotic adjustment in Thermus thermophilus strains HB27 and RQ-1
Authors: Alarico, Susana 
Empadinhas, Nuno 
Mingote, Ana 
Simões, Catarina 
Santos, Maria 
Costa, Milton da 
Issue Date: 2007
Citation: Extremophiles. 11:6 (2007) 833-840
Abstract: Abstract We disrupted the mpgS encoding mannosyl-3-phosphoglycerate synthase (MpgS) of Thermus thermophilus strains HB27 and RQ-1, by homologous recombination, to assess the role of the compatible solute mannosylglycerate (MG) in osmoadaptation of the mutants, to examine their ability to grow in NaCl-containing medium and to identify the intracellular organic solutes. Strain HB27 accumulated only MG when grown in defined medium containing 2% NaCl; mutant HB27M9 did not grow in the same medium containing more than 1% NaCl. When trehalose or MG was added, the mutant was able to grow up to 2% of NaCl and accumulated trehalose or MG, respectively, plus amino acids. T. thermophilus RQ-1 grew in medium containing up to 5% NaCl, accumulated trehalose and lower amounts of MG. Mutant RQ-1M1 lost the ability to grow in medium containing more than 3% NaCl and accumulated trehalose and moderate levels of amino acids. Exogenous MG did not improve the ability of the organism to grow above 3% NaCl, but caused a decrease in the levels of amino acids. Our results show that MG serves as a compatible solute primarily during osmoadaptation at low levels of NaCl while trehalose is primarily involved in osmoadaptation during growth at higher NaCl levels.
URI: http://hdl.handle.net/10316/7819
DOI: 10.1007/s00792-007-0106-x
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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