Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5859
DC FieldValueLanguage
dc.contributor.authorReis, Catarina Pinto-
dc.contributor.authorRibeiro, António J.-
dc.contributor.authorHoung, Simone-
dc.contributor.authorVeiga, Francisco-
dc.contributor.authorNeufeld, Ronald J.-
dc.date.accessioned2008-09-26T17:43:01Z-
dc.date.available2008-09-26T17:43:01Z-
dc.date.issued2007en_US
dc.identifier.citationEuropean Journal of Pharmaceutical Sciences. 30:5 (2007) 392-397en_US
dc.identifier.urihttp://hdl.handle.net/10316/5859-
dc.description.abstractInsulin-loaded alginate-dextran nanospheres were prepared by nanoemulsion dispersion followed by triggered in situ gelation. Nanospheres were characterized for mean size and distribution by laser diffraction spectroscopy and for shape by transmission electron microscopy. Insulin encapsulation efficiency and in vitro release were determined by Bradford protein assay and bioactivity determined in vitro using a newly developed Western blot immunoassay and in vivo using Wistar diabetic rats. Nanospheres ranged from 267 nm to 2.76 [mu]m in diameter and demonstrated a unimodal size distribution. Insulin encapsulation efficiency was 82.5%. Alginate-dextran particles suppressed insulin release in acidic media and promoted a sustained release at near neutral conditions. Nanoencapsulated insulin was bioactive, demonstrated through both in vivo and in vitro bioassaysen_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T25-4MTC6GD-1/1/c26097e450ad242243e8df9feae8643fen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAlginate-dextran nanospheresen_US
dc.subjectNanospheresen_US
dc.subjectInsulin bioactivityen_US
dc.titleNanoparticulate delivery system for insulin: Design, characterization and in vitro/in vivo bioactivityen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
Appears in Collections:FFUC- Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file7fcf745e93324a4cbc35308966cd979f.pdf292.05 kBAdobe PDFView/Open
Show simple item record

Page view(s) 50

333
checked on Aug 12, 2019

Download(s) 50

335
checked on Aug 12, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.