Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/5336
Título: | Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis | Autor: | Oliveira, Helena Loureiro, João Filipe, Luísa Santos, Conceição Ramalho-Santos, João Sousa, Mário Pereira, Maria de Lourdes |
Palavras-chave: | Cadmium chloride; Flow cytometry; Lead chloride; Spermatogenesis; Testicular toxicity | Data: | 2006 | Citação: | Reproductive Toxicology. 22:3 (2006) 529-535 | Resumo: | Flow cytometry (FCM) is a powerful tool to evaluate cell DNA content and ploidy levels. We have assessed the accuracy of two protocols of nuclei isolation from paraffinized samples (P1 and P2) by comparing FCM results with those obtained using fresh material (F1-F3). After isolation, nuclei were stained with propidium iodide and quantitatively analysed by FCM for changes in germ cell ratios. Results obtained with Protocol P2 were similar to those obtained using the protocol that gave best results for fresh tissues (F2). Protocol P2 was then applied to paraffin embedded testicular samples from ICR-CD1 mice exposed to 1, 2 and 3 mg CdCl2/kg bw by single subcutaneous injection, and to 74 and 100 mg PbCl2/kg bw administered in four repeated doses. The highest doses of CdCl2 decreased the number of haploid (1C) cells and increased the number of diploid (2C), S phase and tetraploid (4C) cells. Treatment with PbCl2 did not induce significant changes in testicular cells subpopulations. These results support the usefulness of FCM in evaluating the effect of toxic substances on mouse spermatogenesis, using both fresh and paraffinized material. | URI: | https://hdl.handle.net/10316/5336 | DOI: | 10.1016/j.reprotox.2006.03.009 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
file0917e43e1fee41aa819dddbe8c0bca4b.pdf | 542.49 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
21
Visto em 14/out/2024
Citações WEB OF SCIENCETM
18
Visto em 2/out/2024
Visualizações de página
328
Visto em 15/out/2024
Downloads
330
Visto em 15/out/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.