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Title: Electrochemical detection of in situ adriamycin oxidative damage to DNA
Authors: Oliveira-Brett, A. M. 
Vivan, M. 
Fernandes, I. R. 
Piedade, J. A. P. 
Keywords: Adriamycin; DNA-biosensor; Oxidative damage; 8-Oxoguanine; Voltammetry
Issue Date: 2002
Citation: Talanta. 56:5 (2002) 959-970
Abstract: Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained.
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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