Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5192| Title: | Electrochemical detection of in situ adriamycin oxidative damage to DNA | Authors: | Oliveira-Brett, A. M. Vivan, M. Fernandes, I. R. Piedade, J. A. P. |
Keywords: | Adriamycin; DNA-biosensor; Oxidative damage; 8-Oxoguanine; Voltammetry | Issue Date: | 2002 | Citation: | Talanta. 56:5 (2002) 959-970 | Abstract: | Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained. | URI: | https://hdl.handle.net/10316/5192 | DOI: | 10.1016/S0039-9140(01)00656-7 | Rights: | openAccess |
| Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| file1cc3b0c62ec84011af7d7ac3c95dd182.pdf | 217.2 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.