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https://hdl.handle.net/10316/5066
Title: | Electrochemistry of nanoscale DNA surface films on carbon | Authors: | Oliveira-Brett, A. M. Paquim, A. M. Chiorcea Diculescu, V. C. Piedade, J. A. P. |
Keywords: | Electrochemical DNA-biosensor; Nanoscale; Nanobioelectrochemistry; Surface films; Quercetin; Adriamycin | Issue Date: | 2006 | Citation: | Medical Engineering & Physics. 28:10 (2006) 963-970 | Abstract: | A DNA electrochemical biosensor is an integrated receptor-transducer device. The most important step in the development and manufacture of a sensitive DNA-biosensor for the detection of DNA-drug interactions is the immobilization procedure of the nucleic acid probe on the transducer surface. Magnetic A/C Mode atomic force microscopy (MAC Mode AFM) images in air were used to characterize two different procedures for immobilising nanoscale double-stranded DNA (dsDNA) surface films on carbon electrodes. Thin film dsDNA layers presented holes in the dsDNA film that left parts of the electrode surface uncovered while thicker films showed a uniform and complete coverage of the electrode. These two procedures for preparing dsDNA-biosensors were used to study the influence of reactive oxygen species (ROS) in the mechanism of DNA damage by quercetin, a flavonoid, and adriamycin, an anthracycline anticancer drug. The study of quercetin-DNA interactions in the presence of Cu(II) ions indicated that the formation of a quercetin-Cu(II) complex leads to the formation of ROS necessary to react with DNA, disrupting the helix and causing the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo). Reduced adriamycin radicals are able to directly cause oxidative damage to DNA, generating 8-oxodGuo and ROS are not directly involved in this genomic mutagenic lesion. | URI: | https://hdl.handle.net/10316/5066 | DOI: | 10.1016/j.medengphy.2006.05.009 | Rights: | openAccess |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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