Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5014
Title: Enzyme immobilisation on electroactive nanostructured membranes (ENM): Optimised architectures for biosensing
Authors: Crespilho, Frank N. 
Ghica, M. Emilia 
Gouveia-Caridade, Carla 
Oliveira Jr., Osvaldo N. 
Brett, Christopher M.A. 
Keywords: Layer-by-layer; Glucose oxidase; Gold nanoparticles; PAMAM dendrimer; Cobalt hexacyanoferrate redox mediator
Issue Date: 1-Sep-2008
Citation: Talanta. In Press, Corrected Proof:
Abstract: Electroactive nanostructured membranes have been produced by the layer-by-layer (LbL) technique, and used to make electrochemical enzyme biosensors for glucose by modification with cobalt hexacyanoferrate redox mediator and immobilisation of glucose oxidase enzyme. Indium tin oxide (ITO) glass electrodes were modified with up to three bilayers of polyamidoamine (PAMAM) dendrimers containing gold nanoparticles and poly(vinylsulfonate) (PVS). The gold nanoparticles were covered with cobalt hexacyanoferrate that functioned as a redox mediator, allowing the modified electrode to be used to detect H2O2, the product of the oxidase enzymatic reaction, at 0.0 V vs. SCE. Enzyme was then immobilised by cross-linking with glutaraldehyde. Several parameters for optimisation of the glucose biosensor were investigated, including the number of deposited bilayers, the enzyme immobilisation protocol and the concentrations of immobilised enzyme and of the protein that was crosslinked with PAMAM. The latter was used to provide glucose oxidase with a friendly environment, in order to preserve its bioactivity. The optimised biosensor, with three bilayers, has high sensitivity and operational stability, with a detection limit of 6.1 [mu]M and an apparent Michaelis-Menten constant of 0.20 mM. It showed good selectivity against interferents and is suitable for glucose measurements in natural samples.
URI: http://hdl.handle.net/10316/5014
DOI: 10.1016/j.talanta.2008.04.054
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
filebd480e8948a841d9b7384161465c2d89.pdf1.18 MBAdobe PDFView/Open
Show full item record

WEB OF SCIENCETM
Citations

40
checked on Jan 2, 2021

Page view(s)

296
checked on Jan 21, 2021

Download(s)

132
checked on Jan 21, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.