Please use this identifier to cite or link to this item:
DC FieldValueLanguage
dc.contributor.authorCrespilho, Frank N.-
dc.contributor.authorGhica, M. Emilia-
dc.contributor.authorGouveia-Caridade, Carla-
dc.contributor.authorOliveira Jr., Osvaldo N.-
dc.contributor.authorBrett, Christopher M.A.-
dc.identifier.citationTalanta. In Press, Corrected Proof:en_US
dc.description.abstractElectroactive nanostructured membranes have been produced by the layer-by-layer (LbL) technique, and used to make electrochemical enzyme biosensors for glucose by modification with cobalt hexacyanoferrate redox mediator and immobilisation of glucose oxidase enzyme. Indium tin oxide (ITO) glass electrodes were modified with up to three bilayers of polyamidoamine (PAMAM) dendrimers containing gold nanoparticles and poly(vinylsulfonate) (PVS). The gold nanoparticles were covered with cobalt hexacyanoferrate that functioned as a redox mediator, allowing the modified electrode to be used to detect H2O2, the product of the oxidase enzymatic reaction, at 0.0 V vs. SCE. Enzyme was then immobilised by cross-linking with glutaraldehyde. Several parameters for optimisation of the glucose biosensor were investigated, including the number of deposited bilayers, the enzyme immobilisation protocol and the concentrations of immobilised enzyme and of the protein that was crosslinked with PAMAM. The latter was used to provide glucose oxidase with a friendly environment, in order to preserve its bioactivity. The optimised biosensor, with three bilayers, has high sensitivity and operational stability, with a detection limit of 6.1 [mu]M and an apparent Michaelis-Menten constant of 0.20 mM. It showed good selectivity against interferents and is suitable for glucose measurements in natural samples.en_US
dc.subjectGlucose oxidaseen_US
dc.subjectGold nanoparticlesen_US
dc.subjectPAMAM dendrimeren_US
dc.subjectCobalt hexacyanoferrate redox mediatoren_US
dc.titleEnzyme immobilisation on electroactive nanostructured membranes (ENM): Optimised architectures for biosensingen_US
item.fulltextCom Texto completo-
item.grantfulltextopen- for Mechanical Engineering- for Mechanical Engineering-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
filebd480e8948a841d9b7384161465c2d89.pdf1.18 MBAdobe PDFView/Open
Show simple item record

Page view(s)

checked on Jul 8, 2020


checked on Jul 8, 2020

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.